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Invitrogen
PDX1 Polyclonal Antibody
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FIGURE: 1 / 6
PDX1 Antibody (PA3-830) in ICC/IF
Immunofluorescent analysis of PDX-1 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PDX-1 polyclonal antibody (Product # PA3-830) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). PDX-1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Product Details
PA3-830
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: Q(21) R G P A P E F S A S P P A C(35)
Conjugate
Form
Liquid
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2252208
Product Specific Information
PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms.
PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein.
PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal.
The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended.
Target Information
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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