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Invitrogen
PARP1 Polyclonal Antibody
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FIGURE: 1 / 8
PARP1 Antibody (PA5-16561) in ICC/IF
Immunofluorescence analysis of PARP was performed using 70% confluent log phase HeLa cells treated with 1 uM staurosporine for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PARP (Poly ADP-Ribose Polymerase) Rabbit Polyclonal Antibody (Product # PA5-16561) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain)... View More
Product Details
PA5-16561
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from the C-terminal region of bovine PARP
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985586
Product Specific Information
PA5-16561 was successfully used to detect PARP in WB, IHC and IF applications.
PA5-16561 targets PARP (Poly ADP-Ribose Polymerase) in WB applications and shows reactivity with mouse, Rat, Bovine, and Human samples.
The PA5-16561 immunogen is a synthetic peptide from the C-terminal region of bovine PARP.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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