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Rockland
Orf2 Polyclonal Antibody
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FIGURE: 1 / 1
Orf2 Antibody (600-401-GT3) in WB
Western Blot of Rabbit Anti-Orf2 antibody. Lane 1: Mouse liver whole cell lysate (W10-000-T020) Load: 10 µg per lane. Primary antibody: Orf2 antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP 611-103-122 at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 150 kDa for Orf2.
Product Details
600-401-GT3
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminus of mouse ORF2 protein.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Orf2 is directed against mouse ORF2 near a c-terminus position. A BLAST analysis was used to suggest reactivity with this protein from human and feline based on 100% homology for the immunogen sequence.
Target Information
The multi-functional nuclear protein ORF2p is involved in reverse transcriptase and endonuclease processes. ORF2 elements are epigenetically silenced throughout embyronic development and it is suggested that mutations of L1 would result in detrimental de novo insertions into the genome, negatively affecting gene expression. L1 elements are critical components in the mammalian genome, and much is still not known regarding these proteins. Studies implicate that line-1 is activated in fetal oocytes and L1ORF2p may contribute to the eradication or selection of the oocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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