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Invitrogen
Nhe-1 (extracellular) Polyclonal Antibody
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FIGURE: 1 / 4
Nhe-1 (extracellular) Antibody (PA5-77562) in ICC/IF
Immunocytochemistry analysis of Nhe-1 (extracellular) in human MCF-7 breast adenocarcinoma cells. A) Samples were probed with Nhe-1 (extracellular) polyclonal antibody (Product # PA5-77562) at a dilution of 1:25, and incubated with goat-anti-rabbit-AlexaFluor-594. B) Live view cell image. C) Merged images of Panels A and B.
Product Details
PA5-77562
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide (C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2736220
Product Specific Information
Product is shipped at room temperature as a lyophilized powder and should be stored at -20C upon receipt.
Reconstitute using 50 uL deionized water.
Target Information
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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