Invitrogen

NME2 Polyclonal Antibody

NME2 Antibody in Immunocytochemistry (ICC/IF)

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NME2 Antibody (PA5-15344) in ICC/IF

Immunofluorescent analysis of HeLa cells using a NME2 polyclonal antibody (Product # PA5-15344). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a NME2 polyclonal antibody (Product # PA5-15344) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 1... View More

NME2 Antibody in Immunohistochemistry (IHC)

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NME2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with NME2 siRNA and reduction of signal was observed in Western Blot using NME2 Polyclonal Antibody (Product # PA5-15344). {KD}

Product Details

PA5-15344

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:10-1:50

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NME2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Lot-specific

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2236059

Target Information

Nucleoside diphosphate kinase B is an enzyme that in humans is encoded by the NME2 gene. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein consisting of sequence sharing identity with each individual gene product.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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