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Invitrogen
NDUFS5 Polyclonal Antibody
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FIGURE: 1 / 1
NDUFS5 Antibody (PA5-88682) in WB
Western Blot analysis of NDUFS5 in extracts of various cell lines using NDUFS5 Polyclonal Antibody (Product # PA5-88682) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-88682
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human NDUFS5
Conjugate
Form
Liquid
Concentration
2.03 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805072
Product Specific Information
Immunogen sequence: MPFLDIQKRF GLNIDRWLTI QSGEQPYKMA GRCHAFEKEW IECAHGIGYT RAEKECKIEY DDFVECLLRQ KTMRRAGTIR KQRDKLIKEG KYTPPPHHIG KGEPRP; Positive Samples: LO2, 293T, HL-60, HeLa, SW620, Mouse heart, Mouse kidney, Mouse brain, Rat brain; Cellular Location: Mitochondrion, Mitochondrion inner membrane, Mitochondrion intermembrane space, Peripheral membrane protein
Target Information
Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. By oxidizing NADH that is produced in the Krebs cycle, this complex utilizes the two electrons to reduce ubiquinone to ubiquinol, thereby initiating the passage of electrons to successive complexes and ultimately leading to the reduction of oxygen to water. Mitochondrial complex I consists of over 40 subunits and is of considerable clinical interest since defects in any of the subunits can lead to various myopathies and neuropathies. As a subunit of mitochondrial complex I, NDUFS5 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5), also designated NADH-ubiquinone oxidoreductase 15 kDa subunit, is a 106 amino acid protein that is suggested to not be involved in catalysis. NDUFS5 is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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