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Monkey DNP-albumin Polyclonal Antibody
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Product Details
RAMON-ALBDNP
Product Specifications
Species Reactivity
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Monkey DNP-albumin
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Inter-species cross reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail, however a reaction has been observed with Human albumin, Chicken ovalbuminDNP, but not with native Chicken ovalbumin.
In immunoelectrophoresis use 2 µL serum or equivalent or DNP-conjugates against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter centre well and 2 µL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion (Mancini) use 0.5 to 1% antiserum in the agar gel.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Target Information
Dinitrophenyl is any chemical compound containing two nitro functional groups attached to a phenyl ring. It is a hapten used in vaccine preparation. Dinitrophenyl does not elicit any immune response on its own and it does not bind to any antigen. Albumin is a soluble protein which constitutes approximately 50% of blood serum protein, and acts as a regulator of blood colloidal osmotic pressure and blood volume. Albumin is also an important transporter of substances within the bloodstream, such as fatty acids, thyroid hormones, metal ions and steroids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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