Invitrogen

MiTF Polyclonal Antibody

MiTF Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

MiTF Antibody (PA5-38294) in ICC/IF

Immunofluorescent analysis of MiTF in HeLa cells using a MiTF polyclonal antibody (Product # PA5-38294).

MiTF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-38294

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:100-1:500

View 6 publications 6 publications

Product Specifications

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic non-phosphopeptide derived from human MITF around the phosphorylation site of Ser180/73 (P-N-SP-P-M)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

AB_2554895

Target Information

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts.

STAR protocols

Hood EMS,Curcio CA,Lipinski D

PA5-38294 was used in Immunohistochemistry-immunofluorescence to describe steps for cryoprotecting, cryosectioning, and interrogating with immunofluorescence and histochemistry RPE cells grown on transwell membranes.

Fri Dec 16 00:00:00 UTC 2022

Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts.

STAR protocols

Hood EMS,Curcio CA,Lipinski D

Fri Dec 16 00:00:00 UTC 2022

Higher Mutation Burden in High Proliferation Compartments of Heterogeneous Melanoma Tumors.

International journal of molecular sciences

Grzywa TM,Koppolu AA,Paskal W,Klicka K,Rydzanicz M,Wejman J,Płoski R,Włodarski PK

PA5-38294 was used in Immunohistochemistry to suggest a significant functional role of genetic heterogeneity in melanoma.

Fri Apr 09 00:00:00 UTC 2021

D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degeneration.

Signal transduction and targeted therapy

Wang B,Wang L,Gu S,Yu Y,Huang H,Mo K,Xu H,Zeng F,Xiao Y,Peng L,Liu C,Cao N,Liu Y,Yuan J,Ouyang H

PA5-38294 was used in Immunocytochemistry to describe a high-content screening procedure with a library of 814 protective compounds and found that D609 strongly protected RPE cells from sodium iodate (SI)-induced oxidative cell death and prolonged their healthy survival.

Wed Mar 04 00:00:00 UTC 2020

AKT inhibition-mediated dephosphorylation of TFE3 promotes overactive autophagy independent of MTORC1 in cadmium-exposed bone mesenchymal stem cells.

Autophagy

Pi H,Li M,Zou L,Yang M,Deng P,Fan T,Liu M,Tian L,Tu M,Xie J,Chen M,Li H,Xi Y,Zhang L,He M,Lu Y,Chen C,Zhang T,Wang Z,Yu Z,Gao F,Zhou Z

PA5-38294 was used in Immunocytochemistry to demonstrate that Cd treatment increased autophagic flux and inhibition of the autophagic process using Atg7 gene silencing blocked the Cd-induced mesenchymal stem cell death.

Mon Apr 01 00:00:00 UTC 2019

Suppressing ROS-TFE3-dependent autophagy enhances ivermectin-induced apoptosis in human melanoma cells.

Journal of cellular biochemistry

Deng F,Xu Q,Long J,Xie H

PA5-38294 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that ivermectin increases TFE3-dependent autophagy through ROS signaling pathways in human melanoma cells and that inhibiting autophagy enhances ivermectin-induced apoptosis in human melanoma cells.

Fri Feb 01 00:00:00 UTC 2019

Inhibiting MT2-TFE3-dependent autophagy enhances melatonin-induced apoptosis in tongue squamous cell carcinoma.

Journal of pineal research

Fan T,Pi H,Li M,Ren Z,He Z,Zhu F,Tian L,Tu M,Xie J,Liu M,Li Y,Tan M,Li G,Qing W,Reiter RJ,Yu Z,Wu H,Zhou Z

PA5-38294 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that the melatonin-induced increase in TFE3-dependent autophagy is mediated through the melatonin membrane receptor in tongue squamous cell carcinoma (TSCC).

Thu Mar 01 00:00:00 UTC 2018

Stem Cell Derived Retinal Pigment Epithelium: The Role of Pigmentation as Maturation Marker and Gene Expression Profile Comparison with Human Endogenous Retinal Pigment Epithelium.

Stem cell reviews and reports

Bennis A,Jacobs JG,Catsburg LAE,Ten Brink JB,Koster C,Schlingemann RO,van Meurs J,Gorgels TGMF,Moerland PD,Heine VM,Bergen AA

PA5-38294 was used in Immunocytochemistry-immunoflourescence to discover how pigmentation stages reflect developmental stages and functionality of PSC-derived retinal pigment epithelium cells.

Sun Oct 01 00:00:00 UTC 2017

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