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Invitrogen
MELK Polyclonal Antibody
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FIGURE: 1 / 1
MELK Antibody (PA5-120104) in WB
Western blot analysis of MELK in extracts of various cell lines. Samples were incubated with MELK Polyclonal antibody (Product # PA5-120104) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-120104
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 412-651 of human MELK (NP_055606.1).
Conjugate
Form
Liquid
Concentration
1.83 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913676
Product Specific Information
Positive test controls include: 293T, SKOV3, HT-29, HeLa, B cells, Mouse thymus, Mouse spleen, Rat testis. The target is usually found in the following locations: Cell membrane, Peripheral membrane protein.
Immunogen sequence: LCRTPANKLK NKENVYTPKS AVKNEEYFMF PEPKTPVNKN QHKREILTTP NRYTTPSKAR NQCLKETPIK IPVNSTGTDK LMTGVISPER RCRSVELDLN QAHMEETPKR KGAKVFGSLE RGLDKVITVL TRSKRKGSAR DGPRRLKLHY NVTTTRLVNP DQLLNEIMSI LPKKHVDFVQ KGYTLKCQTQ SDFGKVTMQF ELEVCQLQKP DVVGIRRQRL KGDAWVYKRL VEDILSSCKV
Target Information
MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMELK; hPK38; Maternal embryonic leucine zipper kinase; mPK38; OTTHUMP00000046113; pEg3 kinase; Protein kinase Eg3; Protein kinase PK38; RP11-8N6.1; Tyrosine-protein kinase MELK
Gene Aliases: AI327312; HPK38; KIAA0175; MELK; mKIAA0175; MPK38; Pk38
UniProt ID: (Human) Q14680, (Mouse) Q61846
Entrez Gene ID: (Human) 9833, (Rat) 362510, (Mouse) 17279
protein serine/threonine kinase activity
non-membrane spanning protein tyrosine kinase activity
calcium ion binding
protein binding
ATP binding
lipid binding
protein kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
G2/M transition of mitotic cell cycle
apoptotic process
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
peptidyl-tyrosine phosphorylation
hemopoiesis
intracellular signal transduction
positive regulation of apoptotic process
protein autophosphorylation
neural precursor cell proliferation
protein phosphorylation
cell cycle
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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