Invitrogen

Legionella Polyclonal Antibody

Legionella Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Legionella Antibody (PA1-7227) in ICC/IF

mBio 2014 - FIG 1 PieE inserts in the LCV membrane with its coiled-coil (CC) regions exposed to the cytoplasm. (A) PieE is predicted to contain 4 CC regions and two transmembrane (TM) helices. (B to D) PieE localizes to the LCV in a TM-dependent manner and exposes its CC regions to the cytoplasm. Fluorescent micrographs of A549 cells which were infected with L. pneumophila 130b WT or Delta dotA (T4SS mutant) expressing... View More

Product Image Gallery

Product Details

PA1-7227

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 8 publications 8 publications

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A whole cell preparation of Legionella pneumophila; ATCC #33152.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4-5 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_559903

Product Specific Information

PA1-7227 detects Legionella pneumophila.

PA1-7227 has been successfully used in immunofluorescence procedures.

The PA1-7227 immunogen is a whole cell preparation of Legionella pneumophila; ATCC #33152.

Recommended short term storage of less than 6 months at 2-8°C. For extended storage, aliquot and freeze.

Target Information

Legionella is found in aquatic environments where its growth is supported by amoebae. In human infections, Legionella acts as an intracellular parasite and multiplies in alveolar macrophages. The genus Legionella contains 39 recognized species. The immunological diversity of Legionella is due to the existence of a variety of serogroups. Legionella pneumophila has the most with a total of 14 serogroups.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effector-mediated subversion of proteasome activator (PA)28αβ enhances host defense against Legionella pneumophila under inflammatory and oxidative stress conditions.

PLoS pathogens

Ngwaga T,Chauhan D,Salberg AG,Shames SR

PA1-7227 was used in Immunocytochemistry to evaluate induced proteasome regulators as mediators of innate immunity.

Thu Jun 01 00:00:00 UTC 2023

Intrabacterial Regulation of a Cytotoxic Effector by Its Cognate Metaeffector Promotes Legionella pneumophila Virulence.

mSphere

Chauhan D,Joseph AM,Shames SR

PA1-7227 was used in In vitro Assay to show a novel and important role for translocated effector activity within the bacterium, which challenges the dogma that L. pneumophila effectors function exclusively within host cells.

Tue Feb 21 00:00:00 UTC 2023

Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors.

Life science alliance

Ibe NU,Subramanian A,Mukherjee S

PA1-7227 was used in Sample Preparation to study L. pneumophila toxins activation of UPR sensor ATF6.

Wed Dec 01 00:00:00 UTC 2021

The Legionella pneumophila Metaeffector Lpg2505 (MesI) Regulates SidI-Mediated Translation Inhibition and Novel Glycosyl Hydrolase Activity.

Infection and immunity

Joseph AM,Pohl AE,Ball TJ,Abram TG,Johnson DK,Geisbrecht BV,Shames SR

PA1-7227 was used in Immunocytochemistry-Immunofluorescence to reveal novel enzymatic activity for SidI and provides insight into how intracellular replication of L. pneumophila is regulated by a metaeffector.

Mon Apr 20 00:00:00 UTC 2020

Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.

Cell host & microbe

Jeng EE,Bhadkamkar V,Ibe NU,Gause H,Jiang L,Chan J,Jian R,Jimenez-Morales D,Stevenson E,Krogan NJ,Swaney DL,Snyder MP,Mukherjee S,Bassik MC

PA1-7227 was used in Immunocytochemistry-immunoflourescence to characterize C1orf43 and KIAA1109 as regulators of phagocytosis and show that RAB10 and its chaperone RABIF are required for optimal L. pneumophila replication and ER recruitment to the LCV.

Wed Oct 09 00:00:00 UTC 2019

The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence.

Methods in molecular biology (Clifton, N.J.)

Frankel G,Schroeder GN

PA1-7227 was used in Immunocytochemistry to describe the larvae of the lepidopteran Galleria mellonella as insect model of Legionella pathogenesis.

Thu Jun 20 00:00:00 UTC 2019

The Legionella effector LtpM is a new type of phosphoinositide-activated glucosyltransferase.

The Journal of biological chemistry

Levanova N,Mattheis C,Carson D,To KN,Jank T,Frankel G,Aktories K,Schroeder GN

PA1-7227 was used in Immunocytochemistry to show that purified LtpM nevertheless exhibits glucohydrolase and autoglucosylation activity in vitro and demonstrate that PI3P binding activates LtpM's glucosyltransferase activity toward protein substrates.

Fri Feb 22 00:00:00 UTC 2019

Legionella pneumophila Effector LpdA Is a Palmitoylated Phospholipase D Virulence Factor.

Infection and immunity

Schroeder GN,Aurass P,Oates CV,Tate EW,Hartland EL,Flieger A,Frankel G

PA1-7227 was used in immunocytochemistry to characterize Legionella pneumophilia LpdA

Thu Oct 01 00:00:00 UTC 2015

A new method to determine in vivo interactomes reveals binding of the Legionella pneumophila effector PieE to multiple rab GTPases.

mBio

Mousnier A,Schroeder GN,Stoneham CA,So EC,Garnett JA,Yu L,Matthews SJ,Choudhary JS,Hartland EL,Frankel G

Published figure using Legionella polyclonal antibody (Product # PA1-7227) in Immunofluorescence

Tue Aug 12 00:00:00 UTC 2014

A new method to determine in vivo interactomes reveals binding of the Legionella pneumophila effector PieE to multiple rab GTPases.

mBio

Mousnier A,Schroeder GN,Stoneham CA,So EC,Garnett JA,Yu L,Matthews SJ,Choudhary JS,Hartland EL,Frankel G

Published figure using Legionella polyclonal antibody (Product # PA1-7227) in Immunofluorescence

Tue Aug 12 00:00:00 UTC 2014

The Dot/Icm effector SdhA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Infection and immunity

Harding CR,Stoneham CA,Schuelein R,Newton H,Oates CV,Hartland EL,Schroeder GN,Frankel G

PA1-7227 was used in immunocytochemistry to study the requirement of Legionella pneumophila SdhA for virulence in Galleria mellonella and mice

Mon Jul 01 00:00:00 UTC 2013

LtpD is a novel Legionella pneumophila effector that binds phosphatidylinositol 3-phosphate and inositol monophosphatase IMPA1.

Infection and immunity

Harding CR,Mattheis C,Mousnier A,Oates CV,Hartland EL,Frankel G,Schroeder GN

PA1-7227 was used in immunocytochemistry to study the role of the Legionella pneumophila effector LtpD as a phophoinositide-binding protein involved in bacterial replication

Fri Nov 01 00:00:00 UTC 2013

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Legionella Polyclonal Antibody

Legionella Antibody (PA1-7227) in ICC/IF

Product Details

PA1-7227

Immunocytochemistry (ICC/IF)

In vitro Assay (IV)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

We're here to help

Your items have has been added!