Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Laminin beta-1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
Laminin beta-1 Antibody (PA5-121243) in WB
Western blot analysis of Laminin beta-1 in various lysates. Samples were incubated with Laminin beta-1 Polyclonal antibody (Product # PA5-121243) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-121243
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2).
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914815
Product Specific Information
Positive test controls include: SW480, HepG2, HT-1080, SKOV3, Mouse heart. The target is usually found in the following locations: Secreted, basement membrane, extracellular matrix, extracellular space.
Immunogen sequence: EAKLRADEAK QSAEDILLKT NATKEKMDKS NEELRNLIKQ IRNFLTQDSA DLDSIEAVAN EVLKMEMPST PQQLQNLTED IRERVESLSQ VEVILQHSAA DIARAEMLLE EAKRASKSAT DVKVTADMVK EALEEAEKAQ VAAEKAIKQA DEDIQGTQNL LTSIESETAA SEETLFNASQ RISELERNVE ELKRKAAQNS GEAEYIEKVV YTVKQSAEDV KKTLDGELDE KYKKVENLIA KKTEESADAR RKAEMLQNEA KTLLAQANSK LQLLKDLERK YEDNQRYLED KAQELARLEG EVRSLLKDIS QKVAVYSTCL
Target Information
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support