Invitrogen

LOX Polyclonal Antibody

LOX Antibody in Immunohistochemistry (IHC)

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LOX Antibody (PA1-46020) in IHC

Immunohistochemical analysis of LOX using a polyclonal antibody (Product # PA1-46020).

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LOX Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of LOX was observed in Mouse Heart (Lane 1) and Mouse Kidney (Lane 2) that express LOX protein, in comparison to, Mouse Spleen that doesnt express LOX protein using Anti-LOX Rabbit Polyclonal Antibody (Product # PA1-46020) in Western Blot. {RE}

Product Details

PA1-46020

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:250

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Two synthetic peptides (residues 150-200 and 275-325) of the rat LOX protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2134926

Target Information

Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extra cellular matrix. As well, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

PA1-46020 was used in Immunohistochemistry or Western Blotting to show increased stiffness of pulmonary arteries from patients with left heart disease that correlates with impaired pulmonary hemodynamics.

Fri Jul 21 00:00:00 UTC 2023

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

Fri Jul 21 00:00:00 UTC 2023

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

Fri Jul 21 00:00:00 UTC 2023

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

Fri Jul 21 00:00:00 UTC 2023

Lysyl oxidase-like 2 processing by factor Xa modulates its activity and substrate preference.

Communications biology

Wang H,Poe A,Martinez Yus M,Pak L,Nandakumar K,Santhanam L

PA1-46020 was used in Western Blot to show that Factor Xa (FXa) is a protease that processes LOXL2 at Arg-338.

Fri Apr 07 00:00:00 UTC 2023

High lysyl oxidase expression is an indicator of poor prognosis in dogs with cutaneous mast cell tumours.

Veterinary and comparative oncology

Joselevitch JA,Vargas THM,Pulz LH,Cadrobbi KG,Huete GC,Nishiya AT,Kleeb SR,Xavier JG,Strefezzi RF

PA1-46020 was used in Immunohistochemistry to indicate that high expression of cytoplasmic LOX in neoplastic mast cells is an indicator of poor prognosis for canine cutaneous MCTs.

Fri Sep 01 00:00:00 UTC 2023

An in situ activity assay for lysyl oxidases.

Communications biology

Wang H,Poe A,Pak L,Nandakumar K,Jandu S,Steppan J,Löser R,Santhanam L

PA1-46020 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to develop an in situ activity assay by utilizing the strong reaction between hydrazide and aldehyde to label the LOX-catalyzed allysine (-CHO) residues with biotin-hydrazide.

Mon Jul 05 00:00:00 UTC 2021

An in situ activity assay for lysyl oxidases.

Communications biology

Wang H,Poe A,Pak L,Nandakumar K,Jandu S,Steppan J,Löser R,Santhanam L

Mon Jul 05 00:00:00 UTC 2021

An in situ activity assay for lysyl oxidases.

Communications biology

Wang H,Poe A,Pak L,Nandakumar K,Jandu S,Steppan J,Löser R,Santhanam L

Mon Jul 05 00:00:00 UTC 2021

Acute lysyl oxidase inhibition alters microvascular function in normotensive but not hypertensive men and women.

American journal of physiology. Heart and circulatory physiology

Craighead DH,Wang H,Santhanam L,Alexander LM

PA1-46020 was used in Western Blotting to evaluate the effects of LOX inhibition on cutaneous microvascular function to determine whether LOX function was upregulated in hypertensive humans.

Thu Mar 01 00:00:00 UTC 2018

Immunohistochemistry in Investigative and Toxicologic Pathology.

Toxicologic pathology

Janardhan KS,Jensen H,Clayton NP,Herbert RA

PA1-46020 was used in Immunohistochemistry to review the technical aspects of immunohistochemistry, the variables that affect the results, interpretation, and reproducibility of IHC results.

Sun Jul 01 00:00:00 UTC 2018

Aldosterone-induced cardiomyocyte growth, and fibroblast migration and proliferation are mediated by TRAF3IP2.

Cellular signalling

Somanna NK,Yariswamy M,Garagliano JM,Siebenlist U,Mummidi S,Valente AJ,Chandrasekar B

PA1-46020 was used in western blot to test if Aldo-mediated cardiomycocyte hypertrophy is dependent on TRAF3IP2.

Thu Oct 01 00:00:00 UTC 2015

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LOX Polyclonal Antibody

LOX Antibody (PA1-46020) in IHC

Product Details

PA1-46020

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Target Information

Performance Guarantee

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