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Invitrogen
KIF11 Polyclonal Antibody
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FIGURE: 1 / 2
KIF11 Antibody (PA5-19660) in ICC/IF
Immunofluorescent staining of HeLa cells using PA5-19660, anti-Eg5 antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with TBS-T (20mins), BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nucl... View More
Product Details
PA5-19660
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Human Eg5.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
PA5-19660 targets Eg5 in IF and WB applications and shows reactivity with Human samples.
The PA5-19660 immunogen is synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Human Eg5.
PA5-19660 detects Eg5 which has a predicted molecular weight of approximately 130 kDa.
Target Information
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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