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Invitrogen
KDEL Monoclonal Antibody (1F2), PE
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FIGURE: 1 / 1
KDEL Antibody (MA5-45868) in WB
Western Blot analysis of Human Cervical cancer cells. Lane 2: Anti-KDEL (Product # MA5-45868). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with KDEL monoclonal antibody (Product # MA5-45868) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG:HRP at 1:5,000 for 1 hour at RT. Color Development: ECL solution fo... View More
Product Details
MA5-45868
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F2
Immunogen
KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A 1:1,000 dilution of MA5-45868 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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