Invitrogen

IL-23 p19 Monoclonal Antibody (5B2), eBioscience™

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View all (37) IL-23 p19 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-7233-81

Applications
Tested Dilution
Publications

ELISA (ELISA)

1-4 µg/mL
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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

5B2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_842745

Product Specific Information

Description: The 5B2 antibody reacts with the p19 subunit of mouse IL-23. The 5B2 antibody in combination with the C17.8 antibody yields an improved IL-23 sandwich ELISA with greater sensitivity than was achievable with previous assays. The 5B2 antibody is a non-neutralizing antibody.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN gamma. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN gamma production by naive and memory T cells, as compared to IL-12.

Applications Reported: The anti-mouse p19 5B2 antibody has been reported for use as the capture antibody for a mouse IL-23 sandwich ELISA, when used in conjunction with the anti-p40 C17.8 antibody as the detector.

Applications Tested: The 5B2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-23 (p19/p40) protein levels in combination with the biotinylated (p40-specific) C17.8 antibody (Product # 13-7123-81) for detection and recombinant mouse IL-23 (Product # 14-8231-63) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

For specific neutralization of mouse IL-23 protein activity (with no effect on IL-12 p70), the functional grade purified G23-8 antibody is recommended (Product # 16-7232-81) .

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; MGC79388; p40; RP23-388G23.1

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Gene Aliases: IL-23; Il23a; p19

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UniProt ID: (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 83430

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Function(s)
cytokine activity protein binding interleukin-23 receptor binding
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host immune system process positive regulation of T-helper 1 type immune response inflammatory response immune response positive regulation of activation of JAK2 kinase activity negative regulation of interleukin-10 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell proliferation T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat4 protein positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of memory T cell differentiation innate immune response positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter tissue remodeling defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus positive regulation of neutrophil chemotaxis positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment
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