Leinco Technologies

IL-10 Monoclonal Antibody (JES5-2A5), Functional Grade, In Vivo GOLD™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

I-1152-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Neutralization (Neu)

Assay-dependent
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

JES5-2A5

Immunogen

Purified Recombinant Mouse IL-10

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4°C short term, -80°C long term

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Specificity: Clone JES5-2A5 recognizes mouse IL-10.

Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥95% monomer by analytical SEC.

Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.

This antibody has been tested in the following applications: Western Blotting, Neutralization, CyTOF®.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10

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UniProt ID: (Mouse) P18893

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Entrez Gene ID: (Mouse) 16153

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Function(s)
cytokine activity interleukin-10 receptor binding
Process(es)
negative regulation of cytokine production response to molecule of bacterial origin negative regulation of cytokine secretion involved in immune response negative regulation of chronic inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process inflammatory response immune response regulation of gene expression negative regulation of myeloid dendritic cell activation negative regulation of B cell proliferation negative regulation of interferon-gamma production negative regulation of interleukin-12 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production receptor biosynthetic process negative regulation of tumor necrosis factor biosynthetic process defense response to bacterium defense response to protozoan negative regulation of apoptotic process negative regulation of growth of symbiont in host negative regulation of nitric oxide biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of cytokine secretion negative regulation of inflammatory response negative regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity response to glucocorticoid regulation of sensory perception of pain negative regulation of cytokine activity branching involved in labyrinthine layer morphogenesis cellular response to lipopolysaccharide
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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