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Invitrogen
IFI16 Polyclonal Antibody
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IFI16 Antibody (PA5-39180) in WB
Western blot was performed using Anti-IFI16 Polyclonal Antibody (Product # PA5-39180) and a band at 82 kDa corresponding to IFI16 was observed across the cell lines tested and the expression was increased or detected in HeLa and HUVEC upon IFN gamma and IFN beta treatments respectively. Modified whole cell extracts (1% SDS) (30ug lysate) of HeLa (Lane 1), HeLa treated with IFN gamma (15ng/ml for 16hr) (Lane 2), HUVEC (Lane 3), HUVEC treated with IFN beta (1000 IU/ml for 6hr) (Lane 4), Raji (Lane 5), Ramos (Lane 6), THP-1 (Lane 7), Jurkat (... View More
Product Details
PA5-39180
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide derived from the internal region of human IFI16
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2555772
Product Specific Information
PA5-39180 detects IFI16 in WB application with human samples.
Target Information
IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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