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Invitrogen
IDH3A Polyclonal Antibody
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FIGURE: 1 / 1
IDH3A Antibody (PA1-85126) in WB
Western blot analysis of Isocitrate Dehydrogenase was performed by loading various crude extracts on 4-15% SDS-PAGE gels. One gel was stained with Coomassie and served as a reference gel for protein loading (top image). Proteins from the second gel (bottom image) were transferred to a nylon membrane and blocked with 5% non-fat dry milk in PBST for 2 hours at room temperature. The membrane was probed with an Isocitrate Dehydrogenase polyclonal antibody (Product # PA1-85126) at a dilution of 1:1000 overnight at 4C on a rocking platform, f... View More
Product Details
PA1-85126
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified isocitrate dehydrogenase from porcine heart.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Wet ice
Product Specific Information
PA1-85126 detects Isocitrate Dehydrogenase in many different sample types.
PA1-85126 has been successfully used in Western blot and ELISA applications.
The immunogen used to produce PA1-85126 is from purified isocitrate dehydrogenase from porcine heart.
Store at 4°C or -20°C if preferred.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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