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Invitrogen
His6-DEP-1 Polyclonal Antibody
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His6-DEP-1 Antibody (PA5-78139) in WB
His6-DEP-1 Polyclonal Antibody detects PTPRJ protein by western blot analysis. K562 whole cell extracts and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with His6-DEP-1 Polyclonal Antibody (Product # PA5-78139) diluted at 1:1,000.
Product Details
PA5-78139
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human PTPRJ. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735903
Product Specific Information
Positive Control: K562, K562 membrane extract
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by His6-DEP-1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for His6-DEP-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD148; CD148 antigen; Density-enhanced phosphatase 1; DEP-1; HPTP eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; R-PTP-J; Receptor-type tyrosine-protein phosphatase eta; susceptibility to colon cancer 1, mouse, homolog of
Gene Aliases: CD148; DEP1; HPTPeta; PTPRJ; R-PTP-ETA; SCC1
UniProt ID: (Human) Q12913
Entrez Gene ID: (Human) 5795
negative regulation of cell proliferation
negative regulation of platelet-derived growth factor receptor signaling pathway
regulation of cell adhesion
negative regulation of cell growth
negative regulation of cell migration
peptidyl-tyrosine dephosphorylation
negative regulation of epidermal growth factor receptor signaling pathway
negative regulation of vascular permeability
negative regulation of MAP kinase activity
positive regulation of cell adhesion
platelet-derived growth factor receptor signaling pathway
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive chemotaxis
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
contact inhibition
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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