Invitrogen

High Endothelial Venule Marker Monoclonal Antibody (MECA-79), Alexa Fluor™ 488, eBioscience™

High Endothelial Venule Marker Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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High Endothelial Venule Marker Antibody (53-6036-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil using 20 µg/mL Rat IgM Alexa Fluor® 488 Isotype Control (left) or 20 µg/mL Anti-Human/Mouse High Endothelial Venule Marker Alexa Fluor® 488 (right). Nuclei are counterstained with DAPI.

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Product Details

53-6036-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

20 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MECA-79

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10804391

Product Specific Information

Description: This MECA-79 monoclonal antibody recognizes a sulfated carbohydrate epitope present on human and mouse high endothelial venules (HEVs) called peripheral lymph node addressin (PNAd). This glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer's patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues.

The MECA-79 antibody has been reported to inhibit CD62L (L-selectin)-dependent lymphocyte emigration to HEVs and adhesion in lymph nodes and tonsils.

Applications Reported: This MECA-79 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P).

Applications Tested: This MECA-79 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human tonsil with low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the anitbody be titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HEV peripheral lymph node addressin (PNAd) is a glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer's patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Blockade of the CD93 pathway normalizes tumor vasculature to facilitate drug delivery and immunotherapy.

Science translational medicine

Sun Y,Chen W,Torphy RJ,Yao S,Zhu G,Lin R,Lugano R,Miller EN,Fujiwara Y,Bian L,Zheng L,Anand S,Gao F,Zhang W,Ferrara SE,Goodspeed AE,Dimberg A,Wang XJ,Edil BH,Barnett CC,Schulick RD,Chen L,Zhu Y

53-6036-82 was used in Immunohistochemistry to identify a molecular interaction involved in tumor vascular dysfunction and reveal an approach to promote a favorable tumor microenvironment for therapeutic intervention.

Wed Jul 28 00:00:00 UTC 2021

Intestinal CD11b⁺ B Cells Ameliorate Colitis by Secreting Immunoglobulin A.

Frontiers in immunology

Fu Y,Wang Z,Yu B,Lin Y,Huang E,Liu R,Zhao C,Lu M,Xu W,Liu H,Liu Y,Wang L,Chu Y

53-6036-82 was used in Flow Cytometry to demonstrate that CD11b+ B cells are essential intestinal suppressive immune cells and the primary source of intestinal IgA, which plays an indispensable role in maintaining intestinal homeostasis.

Fri Jan 07 00:00:00 UTC 2022

Stepwise transmigration of T- and B cells through a perivascular channel in high endothelial venules.

Life science alliance

Choe K,Moon J,Lee SY,Song E,Back JH,Song JH,Hyun YM,Uchimura K,Kim P

53-6036-82 was used in Inhibition Assays to suggest that HEV ECs and FRCs with perivascular DCs delicately regulate T- and B-cell entry into peripheral lymph nodes.

Sun Aug 01 00:00:00 UTC 2021

Reprogramming NK cells and macrophages via combined antibody and cytokine therapy primes tumors for elimination by checkpoint blockade.

Cell reports

Wang C,Cui A,Bukenya M,Aung A,Pradhan D,Whittaker CA,Agarwal Y,Thomas A,Liang S,Amlashi P,Suh H,Spranger S,Hacohen N,Irvine DJ

53-6036-82 was used in Immunohistochemistry to demonstrate a single-dose combination treatment (termed AIP) using a pan-tumor-targeting antibody surrogate, half-life-extended interleukin-2 (IL-2), and anti-programmed cell death 1 (PD-1), which primes tumors to respond to subsequent ICB and promotes rejection of large established tumors in mice.

Tue Nov 23 00:00:00 UTC 2021

Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential.

Cell

Huang S,Ziegler CGK,Austin J,Mannoun N,Vukovic M,Ordovas-Montanes J,Shalek AK,von Andrian UH

53-6036-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin) to identify and functionally tested a sensory neuro-immune circuit that is responsive to lymph-borne inflammatory signals.

Thu Jan 21 00:00:00 UTC 2021

Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation.

Oncoimmunology

Lee GJ,Jun Y,Yoo HY,Jeon YK,Lee D,Lee S,Kim J

53-6036-82 was used in Immunohistochemistry (Paraffin) to to describe a murine model which expresses the human RHOA mutant gene product in a T-cell specific manner and develops AITL-like symptoms.

Wed May 13 00:00:00 UTC 2020

TCR-pMHC encounter differentially regulates transcriptomes of tissue-resident CD8 T cells.

European journal of immunology

Yoshizawa A,Bi K,Keskin DB,Zhang G,Reinhold B,Reinherz EL

53-6036-82 was used in Immunohistochemistry (Frozen) to show TCR-pMHC interactions among TR and antigen presenting cells in a tissue milieu strongly impact CD8 T cell biology.

Mon Jan 01 00:00:00 UTC 2018

Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing.

JCI insight

Maarouf OH,Uehara M,Kasinath V,Solhjou Z,Banouni N,Bahmani B,Jiang L,Yilmam OA,Guleria I,Lovitch SB,Grogan JL,Fiorina P,Sage PT,Bromberg JS,McGrath MM,Abdi R

53-6036 was used in ICC/IF to emphasise the critical role of fibroblastic reticular cells in the initiation and repair phases of injury following kidney ischemia-reperfusion injury.

Thu Jul 12 00:00:00 UTC 2018

Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues.

BMC biology

Thiriot A,Perdomo C,Cheng G,Novitzky-Basso I,McArdle S,Kishimoto JK,Barreiro O,Mazo I,Triboulet R,Ley K,Rot A,von Andrian UH

53-6036 was used in Immunohistochemistry-immunofluorescence to characterise DARC (Duffy Antigen Receptor for Chemokines) expression and distribution in the microvasculature of murine tissues.

Fri May 19 00:00:00 UTC 2017

Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.

Scientific reports

Arokiasamy S,Zakian C,Dilliway J,Wang W,Nourshargh S,Voisin MB

53-6036 was used in Immunofluorescence to demonstrate a new role for TNFα as a key regulator of neutrophil trafficking into and within the lymphatic system.

Mon Mar 13 00:00:00 UTC 2017

Meningeal Infiltration of the Spinal Cord by Non-Classically Activated B Cells is Associated with Chronic Disease Course in a Spontaneous B Cell-Dependent Model of CNS Autoimmune Disease.

Frontiers in immunology

Dang AK,Tesfagiorgis Y,Jain RW,Craig HC,Kerfoot SM

53-6036 was used in Immunohistochemistry on frozen tissues to characterise B cell infiltration of the spinal cord in a B cell-dependent spontaneous model of CNS autoimmunity, showing meningeal infiltration of the spinal cord by non-classically activated B cells.

Wed Oct 07 00:00:00 UTC 2015

Mannan induces ROS-regulated, IL-17A-dependent psoriasis arthritis-like disease in mice.

Proceedings of the National Academy of Sciences of the United States of America

Khmaladze Ia,Kelkka T,Guerard S,Wing K,Pizzolla A,Saxena A,Lundqvist K,Holmdahl M,Nandakumar KS,Holmdahl R

53-6036 was used in Immunohistochemistry to study the induction and exacerbation of psoriasis and psoriasis arthritis by exposure to exogenous microbial components.

Tue Sep 02 00:00:00 UTC 2014

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High Endothelial Venule Marker Monoclonal Antibody (MECA-79), Alexa Fluor™ 488, eBioscience™

High Endothelial Venule Marker Antibody (53-6036-82) in IHC (P)

Product Details

53-6036-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Performance Guarantee

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