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Invitrogen
Hexanoyl-Lysine adduct Monoclonal Antibody (5E8), PE
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Hexanoyl-Lysine adduct Antibody (MA5-45784) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with Hexanoyl-Lysine adduct monoclonal antibody (Product # MA5-45784) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG.
Product Details
MA5-45784
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5E8
Immunogen
Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932238
Product Specific Information
A 1:1,000 dilution of MA5-45784 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker which is derived from the oxidation of omega-6 unsaturated fatty acid. HEL may be used as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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