Invitrogen

HTR1B Polyclonal Antibody

HTR1B Antibody in Immunocytochemistry (ICC/IF)

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HTR1B Antibody (PA5-65031) in ICC/IF

Immunofluorescent staining of HTR1B in human cell line A549 shows localization to plasma membrane. Samples were probed using a HTR1B Polyclonal Antibody (Product # PA5-65031).

HTR1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HTR1B Antibody in Immunohistochemistry (IHC)

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HTR1B Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of HTR1B demonstrates antibody specificity. Immunohistochemical analysis of HTR1B using anti-HTR1B Polyclonal Antibody (Product # PA5-65031), shows significant staining of HTR1B in human placenta and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of HTR1B within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-65031

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human HTR1B

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2662744

Product Specific Information

Immunogen sequence: MEEPGAQCAPP PPAGSETWVP QANLSSAPSQ NCSAKDYIYQ DS

Highest antigen sequence identity to the following orthologs - mouse 67%, rat 72%.

Target Information

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase. Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum. In situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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