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Invitrogen
HSPB8 Monoclonal Antibody (3C12-H11), APC
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FIGURE: 1 / 2
HSPB8 Antibody (MA5-45253) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Hsp22 monoclonal antibody (Product # MA5-45253) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Granular layer of the epidermis. Some dermal staining.
Product Details
MA5-45253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3C12-H11
Immunogen
His-tagged human recombinant HSP22
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.4, with 1.033mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931707
Product Specific Information
1 µg/mL of MA5-45253 was sufficient for detection of HSP22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 22kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.
Target Information
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-crystallin C chain; crystallin, alpha C; DHMN 2; E2-induced gene 1 protein; heat shock 22kDa protein 8; heat shock 27kDa protein 8; Heat shock protein; heat shock protein 8; Heat shock protein beta-8; Heat shock protein family B member 8; HMN 2; HSB8; HSP; HSPB 8; HspB8; OTTHUMP00000239768; Protein kinase H11; Small stress protein-like protein HSP22
Gene Aliases: AU018630; AW413033; CMT2L; CRYAC; D5Ucla4; DHMN2; E2IG1; H11; H11K; HMN2; HMN2A; HSP20-like; HSP22; HSPB8; PP1629
UniProt ID: (Human) Q9UJY1, (Rat) Q9EPX0, (Mouse) Q9JK92
Entrez Gene ID: (Human) 26353, (Rat) 113906, (Mouse) 80888
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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