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Invitrogen
HSF2 Polyclonal Antibody
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FIGURE: 1 / 2
HSF2 Antibody (PA5-77831) in ICC/IF
Immunofluorescent analysis of HSF2 in human colon carcinoma cell line (RKO). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with HSF2 polyclonal antibody (Product # PA5-77831) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Rabbit 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) HSF2 Antibody, D) Merged image. Magnification: 60x.
Product Details
PA5-77831
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the mid-protein of Human HSF2 (C-ahx-EGRKSKSKPDKQ)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735922
Product Specific Information
A 1:1,000 dilution of PA5-77831 was sufficient for detection of HSF2 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 90 kDa.
Target Information
Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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