Invitrogen

HSC70 Polyclonal Antibody

View all (39) HSC70 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-95060

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsc70 (563-582aa NDEDKQKILDKCNEIINWLD).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806866

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell, human A549 whole cell, rat liver tissue, mouse liver tissue. IHC: Human Lung Cancer tissue, rat kidney tissue. ICC/IF: U20S cell. Flow: HL-60 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; Heat shock protein family A member 8; HIP; LAP; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; SNC6

View more View less

Gene Aliases: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP71; HSP73; HSPA10; HSPA8; LAP-1; LAP1; NIP71

View more View less

UniProt ID: (Human) P11142, (Rat) P63018, (Mouse) P63017

View more View less

Entrez Gene ID: (Human) 3312, (Rat) 24468, (Mouse) 15481

View more View less

Function(s)
G-protein coupled receptor binding phosphatidylserine binding protein binding ATP binding ATPase activity enzyme binding MHC class II protein complex binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled poly(A) RNA binding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion receptor binding ADP binding nucleotide binding
Process(es)
transcription, DNA-templated mRNA processing protein folding RNA splicing protein refolding negative regulation of transcription, DNA-templated positive regulation of mRNA splicing, via spliceosome chaperone mediated protein folding requiring cofactor regulation of cell cycle chaperone-mediated protein folding clathrin coat disassembly negative regulation of fibril organization mRNA splicing, via spliceosome protein targeting to lysosome response to unfolded protein neurotransmitter secretion cellular response to starvation viral process regulation of protein stability regulation of protein complex assembly regulation of mRNA stability ATP metabolic process regulation of protein complex stability chaperone-mediated autophagy late endosomal microautophagy protein targeting to lysosome involved in chaperone-mediated autophagy chaperone-mediated protein transport involved in chaperone-mediated autophagy cell-cell adhesion regulation of cellular response to heat regulation of protein import chaperone-mediated autophagy translocation complex disassembly regulation of transcription, DNA-templated
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support