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Invitrogen
HIF-2 alpha Polyclonal Antibody
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FIGURE: 1 / 4
HIF-2 alpha Antibody (PA5-19494) in ICC/IF
Immunofluorescent staining of Rat PC12 cells using Product # PA5-19494, anti-HIF2 alpha antibody. The cells were fixed with PFA (4%)for 10 minutes, permabilised PBS-T for 20 minutes and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. A solution of BSA (1%), normal goat serum (10%) and glycine (0.3 M) was used to quench autofluorescence and block non-specific protein-protein in... View More
Product Details
PA5-19494
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to Mouse HIF2-alpha aa 600-700 (internal sequence) KLH
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10981539
Product Specific Information
For Western Blot, this antibody detects a HIF-2-alpha specific band at ~96 kDa and has non-specific bands at 240 kDa,25 kDa and 45 kDa.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with mouse based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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