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Invitrogen
HCMV IL-10 Polyclonal Antibody
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FIGURE: 1 / 1
HCMV IL-10 Antibody (PA5-47082) in Neu
Neutralization of HCMV IL-10 in human peripheral blood lymphocytes (PBL). Samples were incubated in HCMV IL-10 polyclonal antibody (Product # PA5-47082) using a dilution of 2 ng/mL. Recombinant Viral HCMV IL‚10 inhibits LPS-indecued IL-1β secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1β secretion inhibited by Recombinant Viral HCMV IL‚10 is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL‚10 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typica... View More
Product Details
PA5-47082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant viral HCMV IL-10 Ala26-Lys176
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610677
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-10, recombinant canine IL-10, recombinant feline IL-10, recombinant cotton rat IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant human IL-10, and recombinant Epstein-Barr virus encoded viral IL-10 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses. It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection. Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus' advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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