GenScript

HA Tag Monoclonal Antibody (5E11D8), THE™

HA Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

HA Tag Antibody (A01244-100) in ICC/IF

293 cells transfected with N-terminal HA tag protein. Primary antibody: 1 µg/mL THE™ Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244). Secondary antibody: 2 µg/mL Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).

HA Tag Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

A01244-100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

Immunocytochemistry (ICC/IF)

1 µg/mL

Flow Cytometry (Flow)

1 µg/mL

ELISA (ELISA)

0.05-0.2 µg/mL

Immunoprecipitation (IP)

1 µg/mL

Miscellaneous PubMed (MISC)

View 13 publications 13 publications

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5E11D8

Immunogen

A KLH coupled synthetic peptide (YPYDVPDYA)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.

GenScript THE™ HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our THE™ elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Psychological interventions for psychosis in adolescents.

The Cochrane database of systematic reviews

Datta SS,Daruvala R,Kumar A

background: Psychosis is an illness characterised by alterations in thoughts and perceptions resulting in delusions and hallucinations. Psychosis is rare in adolescents but can have serious consequences. Antipsychotic medications are the mainstay treatment, and have been shown to be effective. However, there is emerging evidence on psychological interventions such as cognitive remediation therapy, psycho-education, family therapy and group psychotherapy that may be useful for adolescents with ps

Fri Jul 03 00:00:00 UTC 2020

Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development.

Neuron

Nakagawa N,Plestant C,Yabuno-Nakagawa K,Li J,Lee J,Huang CW,Lee A,Krupa O,Adhikari A,Thompson S,Rhynes T,Arevalo V,Stein JL,Molnár Z,Badache A,Anton ES

Polarized non-overlapping regularly spaced tiled organization of radial glial cells (RGCs) serves as a framework to generate and organize cortical neuronal columns layers and circuitry. Here we show that mediator of cell motility 1 (Memo1) is a critical determinant of radial glial tiling during neocortical development. Memo1 deletion or knockdown leads to hyperbranching of RGC basal processes and disrupted RGC tiling resulting in aberrant radial unit assembly and neuronal layering. Memo1 regulat

Wed Sep 04 00:00:00 UTC 2019

Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS.

Nature microbiology

Ghosal D,Jeong KC,Chang YW,Gyore J,Teng L,Gardner A,Vogel JP,Jensen GJ

Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Here, we used complementary electron cryotomography and immunofluorescence microscopy to investigate the molecular architecture and biogenesis of the Dot/Icm secretion apparatus. Electron cryotomography mapped the location of the core and accessory components of the Legionella core t

Mon Jul 01 00:00:00 UTC 2019

Substrate relay in an Hsp70-cochaperone cascade safeguards tail-anchored membrane protein targeting.

The EMBO journal

Cho H,Shan SO

Membrane proteins are aggregation-prone in aqueous environments and their biogenesis poses acute challenges to cellular protein homeostasis. How the chaperone network effectively protects integral membrane proteins during their post-translational targeting is not well understood. Here biochemical reconstitutions showed that the yeast cytosolic Hsp70 is responsible for capturing newly synthesized tail-anchored membrane proteins (TAs) in the soluble form. Moreover direct interaction of Hsp70 with

Wed Aug 15 00:00:00 UTC 2018

Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF₁₆₅ Neutralization.

Marine drugs

Camacho-Villegas TA,Mata-González MT,García-Ubbelohd W,Núñez-García L,Elosua C,Paniagua-Solis JF,Licea-Navarro AF

Variable new antigen receptor domain (vNAR) antibodies are novel naturally occurring antibodies that can be isolated from naïve immune or synthetic shark libraries. These molecules are very interesting to the biotechnology and pharmaceutical industries because of their unique characteristics related to size and tissue penetrability. There have been some approved anti-angiogenic therapies for ophthalmic conditions not related to vNAR. This includes biologics and chimeric proteins that neutra

Sat Mar 31 00:00:00 UTC 2018

Rapid generation of hypomorphic mutations.

Nature communications

Arthur LL,Chung JJ,Jankirama P,Keefer KM,Kolotilin I,Pavlovic-Djuranovic S,Chalker DL,Grbic V,Green R,Menassa R,True HL,Skeath JB,Djuranovic S

Hypomorphic mutations are a valuable tool for both genetic analysis of gene function and for synthetic biology applications. However current methods to generate hypomorphic mutations are limited to a specific organism change gene expression unpredictably or depend on changes in spatial-temporal expression of the targeted gene. Here we present a simple and predictable method to generate hypomorphic mutations in model organisms by targeting translation elongation. Adding consecutive adenosine nucl

Fri Jan 20 00:00:00 UTC 2017

Internal modifications in the CENP-A nucleosome modulate centromeric dynamics.

Epigenetics & chromatin

Bui M,Pitman M,Nuccio A,Roque S,Donlin-Asp PG,Nita-Lazar A,Papoian GA,Dalal Y

Mon Jan 29 00:00:00 UTC 2018

NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.

Nature

He Y,Zeng MY,Yang D,Motro B,Núñez G

Inflammasomes are intracellular protein complexes that drive the activation of inflammatory caspases. So far, four inflammasomes involving NLRP1, NLRP3, NLRC4 and AIM2 have been described that recruit the common adaptor protein ASC to activate caspase-1, leading to the secretion of mature IL-1β and IL-18 proteins. The NLRP3 inflammasome has been implicated in the pathogenesis of several acquired inflammatory diseases as well as cryopyrin-associated periodic fever syndromes (CAPS) caused by

Thu Feb 18 00:00:00 UTC 2016

Establishment of an inducible HBV stable cell line that expresses cccDNA-dependent epitope-tagged HBeAg for screening of cccDNA modulators.

Antiviral research

Cai D,Wang X,Yan R,Mao R,Liu Y,Ji C,Cuconati A,Guo H

Hepatitis B virus (HBV) covalently closed circular (ccc) DNA is essential to the virus life cycle, its elimination during chronic infection is considered critical to a durable therapy but has not been achieved by current antivirals. Despite being essential, cccDNA has not been the major target of high throughput screening (HTS), largely because of the limitations of current HBV tissue culture systems, including the impracticality of detecting cccDNA itself. In response to this need, we have prev

Mon Aug 01 00:00:00 UTC 2016

Modulation of hepatitis B surface antigen secretion by annexin II expressed in hepatitis B virus‑producing hepatoma cells.

Molecular medicine reports

Liu Y,Gao P

The role of annexin II in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) remains to be elucidated. Intracellular hepatitis B surface antigen (HBsAg)-retention contributes to the induction of hepatocarcinogenesis. The present study aimed to investigate the regulation of HBsAg secretion by annexin II expressed in HBV-producing hepatoma cells. The expression of annexin II was analyzed using western blot analysis in SMMC-7721, HepG2, HepG2.2.15, 293T and Chinese hamster ovary (CHO

Mon Dec 01 00:00:00 UTC 2014

Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.

Nature methods

Swaney DL,Beltrao P,Starita L,Guo A,Rush J,Fields S,Krogan NJ,Villén J

Cross-talk between different types of post-translational modifications on the same protein molecule adds specificity and combinatorial logic to signal processing, but it has not been characterized on a large-scale basis. We developed two methods to identify protein isoforms that are both phosphorylated and ubiquitylated in the yeast Saccharomyces cerevisiae, identifying 466 proteins with 2,100 phosphorylation sites co-occurring with 2,189 ubiquitylation sites. We applied these methods quantitati

Mon Jul 01 00:00:00 UTC 2013

Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator.

The Journal of biological chemistry

Shulga YV,Anderson RA,Topham MK,Epand RM

Phosphatidylinositol 4,5-bisphosphate is mostly produced in the cell by phosphatidylinositol-4-phosphate 5-kinases (PIP5K) and has a crucial role in numerous signaling events. Here we demonstrate that in vitro all three isoforms of PIP5K, α, β, and γ, discriminate among substrates with different acyl chains for both the substrates phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol (PtdIns) although to different extents, with isoform γ being the most selec

Fri Oct 19 00:00:00 UTC 2012

Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Borroto-Escuela DO,Romero-Fernandez W,García-Negredo G,Correia PA,Garriga P,Fuxe K,Ciruela F

Acetylcholine challenge produces M3 muscarinic acetylcholine receptor activation and accessory/scaffold proteins recruitment into a signalsome complex. The dynamics of such a complex is not well understood but a conserved NPxxY motif located within transmembrane 7 and juxtamembrane helix 8 of the receptor was found to modulate G protein activation. Here by means of receptor mutagenesis we unravel the role of the conserved M3 muscarinic acetylcholine receptor NPxxY motif on ligand binding, signal

Fri Apr 20 00:00:00 UTC 2012

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HA Tag Monoclonal Antibody (5E11D8), THE™

HA Tag Antibody (A01244-100) in ICC/IF

Product Details

A01244-100

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Performance Guarantee

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