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Invitrogen
GRP94 Monoclonal Antibody (6B12), FITC
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FIGURE: 1 / 1
GRP94 Antibody (MA5-45878) in WB
Western Blot analysis of Human Cervical cancer cells. Lane 2: HeLa cell lysates (10 µg). Load: 10 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with GRP94 monoclonal antibody (Product # MA5-45878) at 1:1,000 for O/N at 4°C, followed by Goat Anti-Mouse IgG:HRP at 1:3,000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 94 kDa.
Product Details
MA5-45878
Product Specifications
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6B12
Immunogen
KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A 1:1,000 dilution of MA5-45878 was sufficient for detection of GRP94 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 94 kDa band corresponding to GRP94.
Target Information
GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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