Invitrogen

GPX1 Polyclonal Antibody

GPX1 Antibody in Immunocytochemistry (ICC/IF)

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GPX1 Antibody (PA5-30593) in ICC/IF

Immunofluorescent analysis of GPX1 in paraformaldehyde-fixed HeLa cells using a GPX1 polyclonal antibody (Product # PA5-30593) at a 1:200 dilution.

GPX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-30593

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Published species

Cynomolgus monkey, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 127 and 203 of Human GPX1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.14 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2548067

Product Specific Information

Recommended positive controls: Molt-4, Raji, mouse liver, GL261, C8D30, NIH-3T3, Raw264.7, C2C12.

Predicted reactivity: Rhesus Monkey (100%), Chimpanzee (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impact of Roux-en-Y Gastric Bypass on Mitochondrial Biogenesis and Dynamics in Leukocytes of Obese Women.

Antioxidants (Basel, Switzerland)

Abad-Jiménez Z,Vezza T,López-Domènech S,Fernández-Reyes M,Canet F,Morillas C,Gómez-Abril SÁ,Bañuls C,Víctor VM,Rocha M

PA5-30593 was used in Western Blot to investigate whether RYGB modulates oxidative stress, inflammation and mitochondrial dynamics in the leukocytes of 47 obese women at one year follow-up.

Wed Jun 29 00:00:00 UTC 2022

MG53 suppresses NF-κB activation to mitigate age-related heart failure.

JCI insight

Wang X,Li X,Ong H,Tan T,Park KH,Bian Z,Zou X,Haggard E,Janssen PM,Merritt RE,Pawlik TM,Whitson BA,Mokadam NA,Cao L,Zhu H,Cai C,Ma J

Published figure using GPX1 polyclonal antibody (Product # PA5-30593) in Western Blot

Wed Sep 08 00:00:00 UTC 2021

Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.

Cell

Wang S,Zheng Y,Li J,Yu Y,Zhang W,Song M,Liu Z,Min Z,Hu H,Jing Y,He X,Sun L,Ma L,Esteban CR,Chan P,Qiao J,Zhou Q,Izpisua Belmonte JC,Qu J,Tang F,Liu GH

PA5-30593 was used in Immunohistochemistry to identify distinct gene-expression signatures in ovarian cell types in non-human primates.

Thu Feb 06 00:00:00 UTC 2020

PDGF-BB Preserves Mitochondrial Morphology, Attenuates ROS Production, and Upregulates Neuroglobin in an Astrocytic Model Under Rotenone Insult.

Molecular neurobiology

Cabezas R,Vega-Vela NE,González-Sanmiguel J,González J,Esquinas P,Echeverria V,Barreto GE

PA530593 was used in western blot to discuss mechanisms associated with platelet-derived growth factor, subtype BB protection against oxidative damage

Sun Apr 01 00:00:00 UTC 2018

Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin.

Free radical biology & medicine

Patche J,Girard D,Catan A,Boyer F,Dobi A,Planesse C,Diotel N,Guerin-Dubourg A,Baret P,Bravo SB,Paradela-Dobarro B,Álvarez E,Essop MF,Meilhac O,Bourdon E,Rondeau P

PA530593 was used in western blot to explore the links between oxidative perturbations and plasma albumin modifications in the liver

Sun Jan 01 00:00:00 UTC 2017

Role of tRNA selenocysteine 1 associated protein 1 in the proliferation and apoptosis of cardiomyocyte‑like H9c2 cells.

Molecular medicine reports

Li MD,Cheng WP,Shi MX,Ge TD,Zheng XL,Wu DY,Hu XY,Luo JC,Li FL,Li H

PA5-30593 was used in Western Blotting to study the mechanisms underlying myocardial injury induced by selenium deficiency.

Wed Feb 01 00:00:00 UTC 2017

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