Invitrogen

GPR30 Polyclonal Antibody

GPR30 Antibody in Immunocytochemistry (ICC/IF)

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GPR30 Antibody (PA5-77396) in ICC/IF

Immunofluorescence analysis of GPR30 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GPR30 Polyclonal Antibody (Product # PA5-77396) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034) at ... View More

GPR30 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GPR30 Antibody — (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER

Product Details

PA5-77396

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

AB_2735876

Product Specific Information

Product is shipped at room temperature as a lyophilized powder and should be stored at -20 °C upon receipt. Reconstitution: add 50 µL of deionized water.

Target Information

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

17β-Estradiol promotes sex-specific dysfunction in isolated human arterioles.

American journal of physiology. Heart and circulatory physiology

SenthilKumar G,Katunaric B,Bordas-Murphy H,Young M,Doren EL,Schulz ME,Widlansky ME,Freed JK

PA5-77396 was used in Immunohistochemistry to show that isolated human arterioles from females across the life span maintain nitric oxide (NO)-mediated dilation to flow, whereas chronic (16-20 h) exposure to exogenous (100 nM) 17β-estradiol promotes microvascular endothelial dysfunction in vessels from adult females of 40 and ≥40 yr of age.

Wed Mar 01 00:00:00 UTC 2023

Supraphysiologic doses of 17β-estradiol aggravate depression-like behaviors in ovariectomized mice possibly via regulating microglial responses and brain glycerophospholipid metabolism.

Journal of neuroinflammation

Li M,Zhang J,Chen W,Liu S,Liu X,Ning Y,Cao Y,Zhao Y

PA5-77396 was used in Immunohistochemistry-immunofluorescence to suggest that sE2 may be nonhelpful or even detrimental to menopause-related depression, at least partly, by regulating microglial responses and glycerophospholipid metabolism.

Thu Sep 07 00:00:00 UTC 2023

Essential and sex-specific effects of mGluR5 in ventromedial hypothalamus regulating estrogen signaling and glucose balance.

Proceedings of the National Academy of Sciences of the United States of America

Fagan MP,Ameroso D,Meng A,Rock A,Maguire J,Rios M

PA5-77396 was used in Western Blotting to show that mGluR5 depletion in SF1 neurons in female but not in male mice leads to reduced activity of these cells as well as deficient glucose and lipid balance control.

Tue Aug 11 00:00:00 UTC 2020

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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