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Invitrogen
GNAI1 Polyclonal Antibody
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FIGURE: 1 / 6
GNAI1 Antibody (PA5-30043) in ICC/IF
Immunofluorescence analysis of GNAI1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GNAI1 Polyclonal Antibody (Product # PA5-30043) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More
Product Details
PA5-30043
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 132 and 318 of Human G protein alpha Inhibitor 1
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547517
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji.
Predicted reactivity: Human (98%), Mouse (98%), Rat (98%), Zebrafish (97%), Xenopus laevis (97%), Pig (98%), Chicken (97%), Bovine (98%), Guinea pig (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain; and transducin-1 and transducin-2, proteins involved in phototransduction in retinal rods and cones, respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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