Invitrogen

GLUT1 Polyclonal Antibody

GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GLUT1 Antibody (PA1-21041) in IHC (P)

Immunohistochemical analysis of Glucose Transporter 1 in formalin-fixed, paraffin-embedded human esophagous using a Glucose Transporter 1 polyclonal antibody (Product # PA1-21041).

GLUT1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-21041

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide to the C-terminal region of human GLUT1. The peptide sequence is considered commercially sensitive.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C, do not freeze

Shipping conditions

Wet ice

AB_559285

Product Specific Information

PA1-21041 detects glucose transporter (GLUT1) in human and rat samples.

PA1-21041 has been successfully used in immunofluorescenceand immunohistochemistry (paraffin) procedures. Staining of formalin fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 minutes.

PA1-21041 immunogen corresponds to a synthetic peptide to the C-terminal region of human GLUT1. The peptide sequence is considered commercially sensitive.

Target Information

Glucose transporter 1 (GLUT1), encoded by the SLC2A1 gene, is a facilitative glucose transporter that plays a pivotal role in the uptake of glucose across cell membranes, particularly in tissues with high-energy demands such as the brain, erythrocytes, and endothelial cells of the blood-brain barrier. GLUT1 is ubiquitously expressed and functions through a passive diffusion mechanism, allowing glucose to move down its concentration gradient. Its involvement in maintaining glucose homeostasis is crucial for cellular metabolism and energy production. Mutations in SLC2A1 can lead to GLUT1 deficiency syndrome, characterized by seizures, developmental delay, and movement disorders due to impaired glucose transport into the brain. GLUT1 also plays a significant role in cancer biology, where its overexpression is commonly observed in various tumors, facilitating the enhanced glucose uptake required for rapid tumor cell proliferation. This characteristic makes GLUT1 a potential target for cancer diagnostics and therapeutics. Additionally, GLUT1's regulation is influenced by factors such as hypoxia, insulin, and cellular stress, highlighting its adaptive role in different physiological and pathological states.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of capillary pericytes and the blood-brain barrier in white matter in poststroke and vascular dementias and Alzheimer's disease.

Brain pathology (Zurich, Switzerland)

Ding R,Hase Y,Ameen-Ali KE,Ndung'u M,Stevenson W,Barsby J,Gourlay R,Akinyemi T,Akinyemi R,Uemura MT,Polvikoski T,Mukaetova-Ladinska E,Ihara M,Kalaria RN

Published figure using GLUT1 polyclonal antibody (Product # PA1-21041) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2020

Development of passive CLARITY and immunofluorescent labelling of multiple proteins in human cerebellum: understanding mechanisms of neurodegeneration in mitochondrial disease.

Scientific reports

Phillips J,Laude A,Lightowlers R,Morris CM,Turnbull DM,Lax NZ

PA1-21041 was used in immunohistochemistry to use CLARITY to study mechanisms of neurodegeneration that occur in mitochondrial disease

Mon May 16 00:00:00 UTC 2016

Immunolocalization of platelet-derived growth factor receptor-β (PDGFR-β) and pericytes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Neuropathology and applied neurobiology

Craggs LJ,Fenwick R,Oakley AE,Ihara M,Kalaria RN

PA1-21041 was used in western blot to determine the pericyte distribution in relation to N3ECD deposits in cerebral microvessels in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy

Mon Jun 01 00:00:00 UTC 2015

Immunolocalization of platelet-derived growth factor receptor-β (PDGFR-β) and pericytes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Neuropathology and applied neurobiology

Craggs LJ,Fenwick R,Oakley AE,Ihara M,Kalaria RN

Mon Jun 01 00:00:00 UTC 2015

Brain microvascular accumulation and distribution of the NOTCH3 ectodomain and granular osmiophilic material in CADASIL.

Journal of neuropathology and experimental neurology

Yamamoto Y,Craggs LJ,Watanabe A,Booth T,Attems J,Low RW,Oakley AE,Kalaria RN

PA1-21041 was used in immunohistochemistry to study the microvascular distribution of the Notch3 ectodomain and granular osmiophilic material in the brains of patients with CADASIL

Wed May 01 00:00:00 UTC 2013

Examination of glucose transporter-1, transforming growth factor-β and neuroglobin immunoreactivity in the orbitofrontal cortex in late-life depression.

Psychiatry and clinical neurosciences

Khundakar A,Morris C,Slade J,Thomas AJ

PA1-21041 was used in immunohistochemistry to identify proteins involved in hypoxic/ischemic signaling in orbitofrontal cortex

Tue Mar 01 00:00:00 UTC 2011

Adenomatoid tumors of the female and male genital tract. A comparative clinicopathologic and immunohistochemical analysis of 47 cases emphasizing their site-specific morphologic diversity.

Virchows Archiv : an international journal of pathology

Wachter DL,Wünsch PH,Hartmann A,Agaimy A

PA1-21041 was used in immunohistochemistry to characterize human genital tract tumor

Sun May 01 00:00:00 UTC 2011

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