Invitrogen

GLT-1 Polyclonal Antibody

FIGURE: 1 / 5

GLT-1 Antibody (PA3-040A) in WB

Western blot detection of GLT-1 from rat brain (lane 2) for your review. Lane 1 is a control illustrating specificity by peptide blocking.

Product Image Gallery

GLT-1 Antibody — Figure 4 Analysis of glucose and glutamate transporters in response to chronic acyl-ghrelin treatment. Hypothalamic levels of ( A ) glucose transporter (GLUT)2, ( B ) GLUT3, ( C ) glial glutamate transporter 1 (GLT1) and ( D ) glutamate-aspartate transporter (GLAST) in male rats receiving acyl-ghrelin (AG) icv for 2 weeks (5 mug/day). Controls received saline and one group of AG treated rats were pair-fed (pf) with controls. *p < 0.05, # p < 0.01. {TM}

Product Details

PA3-040A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues N(556) G K S A D C S V E E E P W K R E K(573) or rat GLT-1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2190755

Product Specific Information

PA3-040A detects glutamate transporter 1 (GLT-1) from rat tissues.

PA3-040A has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ~61 kDa band representing GLT-1 from rat brain extract. This antibody may also detect one or more non-specific bands below 50 kDa that have not been identified.

The PA3-040A immunogen is a synthetic peptide corresponding to residues N(556) G K S A D C S V E E E P W K R E K(573) or rat GLT-1. While GLT-1, GLAST and EAAC1 share approximately 50% sequence homology, the sequence used to generate this product is unique to GLT-1.

Target Information

Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ghrelin Regulates Glucose and Glutamate Transporters in Hypothalamic Astrocytes.

Scientific reports

Fuente-Martín E,García-Cáceres C,Argente-Arizón P,Díaz F,Granado M,Freire-Regatillo A,Castro-González D,Ceballos ML,Frago LM,Dickson SL,Argente J,Chowen JA

PA3-040A was used in western blot to study how glucose and glutamate transporters are regulated by Ghrelin in hypothalamic astrocytes

Wed Mar 30 00:00:00 UTC 2016

Purkinje neuron Ca2+ influx reduction rescues ataxia in SCA28 model.

The Journal of clinical investigation

Maltecca F,Baseggio E,Consolato F,Mazza D,Podini P,Young SM,Drago I,Bahr BA,Puliti A,Codazzi F,Quattrini A,Casari G

PA3-040A was used in western blot to explore the role of calcium handling in SCA28 pathogenesis

Thu Jan 01 00:00:00 UTC 2015

Sumoylation of the astroglial glutamate transporter EAAT2 governs its intracellular compartmentalization.

Glia

Foran E,Rosenblum L,Bogush A,Pasinelli P,Trotti D

PA3-040A was used in immunoprecipitation to study the role of sumoylation in determining the subcellular compartmentalization of the EAAT2 astrogial glutamate transporter

Fri Aug 01 00:00:00 UTC 2014