Invitrogen

FIS1 Polyclonal Antibody

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FIS1 Antibody (PA1-41082) in WB

Knockdown validation of FIS1 in A) human HeLa and B) mouse NIH 3T3 lysate. Samples were incubated in FIS1 polyclonal (Product # PA1-41082) using a dilution of 1:1000. B. Specificity validation of the Fis1 antibody. Fis1 was analyzed in M17 cells stably transfected with vector only, Fis1 RNAi, Fis1 (Fis1wt OE) or left untransfected (negative control). The western blot data showed that M17 cells expressed Fis1 (Vector, Untransfected control) which could be knocked down by Fis1 RNAi. Cells transfected with Fis1 overexpressed Fis1. M17 is a hu... View More

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Product Details

PA1-41082

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

Assay-Dependent

ELISA (ELISA)

Assay-Dependent

Product Specifications

Published species

Cynomolgus monkey, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 11-25 of human Fis1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2102527

Product Specific Information

This antibody is predicted to react with rat, chimp, and canine based on sequence homology.

Suggested positive control: antigen standard for FIS1 (transient overexpression lysate), 3T3 or Hela whole cell lysate.

Target Information

Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans.

Metabolism: clinical and experimental

Axelrod CL,Fealy CE,Erickson ML,Davuluri G,Fujioka H,Dantas WS,Huang E,Pergola K,Mey JT,King WT,Mulya A,Hsia D,Burguera B,Tandler B,Hoppel CL,Kirwan JP

PA1-41082 was used in Western Blotting to indicate that activation of mitochondrial fission and quality control occur early in the onset of insulin resistance in human skeletal muscle.

Sun Aug 01 00:00:00 UTC 2021

Cells Lacking PA200 Adapt to Mitochondrial Dysfunction by Enhancing Glycolysis via Distinct Opa1 Processing.

International journal of molecular sciences

Douida A,Batista F,Boto P,Regdon Z,Robaszkiewicz A,Tar K

Published figure using FIS1 polyclonal antibody (Product # PA1-41082) in Western Blot

Fri Feb 05 00:00:00 UTC 2021

Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells.

Cells

Kim D,Sankaramoorthy A,Roy S

PA1-41082 was used in Western Blotting to investigate whether downregulation of mitochondrial fission genes, Fis1 and Drp1, which are overexpressed in HG condition, prevents mitochondrial fragmentation, preserves mitochondrial function, and protects retinal endothelial cells from apoptosis.

Fri Jul 10 00:00:00 UTC 2020

Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells.

Cells

Kim D,Sankaramoorthy A,Roy S

Fri Jul 10 00:00:00 UTC 2020

Abnormal Mitochondria in a Non-human Primate Model of MPTP-induced Parkinson's Disease: Drp1 and CDK5/p25 Signaling.

Experimental neurobiology

Park J,Seo J,Won J,Yeo HG,Ahn YJ,Kim K,Jin YB,Koo BS,Lim KS,Jeong KJ,Kang P,Lee HY,Baek SH,Jeon CY,Hong JJ,Huh JW,Kim YH,Park SJ,Kim SU,Lee DS,Lee SR,Lee Y

PA1-41082 was used in Western Blotting to suggest that the phosphorylation of Drp1(S616) by CDK5 is involved in mitochondrial fission in the substantia nigra of MPTP-induced Parkinson's Disease in a non-human primate model.

Sat Jun 01 00:00:00 UTC 2019

Exercise training remodels human skeletal muscle mitochondrial fission and fusion machinery towards a pro-elongation phenotype.

Acta physiologica (Oxford, England)

Axelrod CL,Fealy CE,Mulya A,Kirwan JP

PA1-41082 was used in Western Blot to investigate the effect of exercise training on muscle mitochondrial dynamics.

Mon Apr 01 00:00:00 UTC 2019

Loss of succinyl-CoA synthase ADP-forming β subunit disrupts mtDNA stability and mitochondrial dynamics in neurons.

Scientific reports

Zhao Y,Tian J,Sui S,Yuan X,Chen H,Qu C,Du Y,Guo L,Du H

Published figure using FIS1 polyclonal antibody (Product # PA1-41082) in Western Blot

Wed Aug 02 00:00:00 UTC 2017

Autophagy signaling in skeletal muscle of infarcted rats.

PloS one

Jannig PR,Moreira JB,Bechara LR,Bozi LH,Bacurau AV,Monteiro AW,Dourado PM,Wisløff U,Brum PC

PA1-41082 was used in western blot to study skeletal muscle atrophy induced by myocardial infarction and the role of autophagy signaling

Fri Oct 17 00:00:00 UTC 2014

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