Invitrogen

FBXO5 Monoclonal Antibody (3D2D6)

23 Published Figures

21 References

View all (10) FBXO5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (23)

FIGURE: 1 / 24

FBXO5 Antibody (37-6600) in WB

Western blot analysis of HEK293 cell lysates using Ms anti-Emi1 (Product # 37-6600).

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Product Details

37-6600

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3D2D6

Immunogen

Synthetic peptide derived from the C-terminal region of human Emi1 (early mitotic inhibitor 1, Fbx5) protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533333

Target Information

F-box proteins, critical components of the conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oocytes mount a noncanonical DNA damage response involving APC-Cdh1-mediated proteolysis.

The Journal of cell biology

Subramanian GN,Greaney J,Wei Z,Becherel O,Lavin M,Homer HA

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Mon Apr 06 00:00:00 UTC 2020

Oocytes mount a noncanonical DNA damage response involving APC-Cdh1-mediated proteolysis.

The Journal of cell biology

Subramanian GN,Greaney J,Wei Z,Becherel O,Lavin M,Homer HA

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Mon Apr 06 00:00:00 UTC 2020

Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Is Independent of Anaphase-Promoting Complex Activity.

Journal of virology

Elbasani E,Gramolelli S,Günther T,Gabaev I,Grundhoff A,Ojala PM

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Tue Jun 16 00:00:00 UTC 2020

Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Is Independent of Anaphase-Promoting Complex Activity.

Journal of virology

Elbasani E,Gramolelli S,Günther T,Gabaev I,Grundhoff A,Ojala PM

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Tue Jun 16 00:00:00 UTC 2020

Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell-Cycle Exit after DNA Damage.

Cell reports

Segeren HA,van Rijnberk LM,Moreno E,Riemers FM,van Liere EA,Yuan R,Wubbolts R,de Bruin A,Westendorp B

37-6600 was used in Western Blotting to study neoplastic cell expression of E2F-dependent transcription within human cancer biopsy specimens.

Tue Dec 01 00:00:00 UTC 2020

Altered G1 signaling order and commitment point in cells proliferating without CDK4/6 activity.

Nature communications

Liu C,Konagaya Y,Chung M,Daigh LH,Fan Y,Yang HW,Terai K,Matsuda M,Meyer T

37-6600 was used in Western Blotting to delineate sequential molecular events in the CDK2-initiated path that are distinct from the CDK4/6-initiated path.

Tue Oct 20 00:00:00 UTC 2020

EMI1 switches from being a substrate to an inhibitor of APC/C^CDH1 to start the cell cycle.

Nature

Cappell SD,Mark KG,Garbett D,Pack LR,Rape M,Meyer T

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Flow Cytometry

Fri Jun 01 00:00:00 UTC 2018

EMI1 switches from being a substrate to an inhibitor of APC/C^CDH1 to start the cell cycle.

Nature

Cappell SD,Mark KG,Garbett D,Pack LR,Rape M,Meyer T

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Flow Cytometry

Fri Jun 01 00:00:00 UTC 2018

EMI1 switches from being a substrate to an inhibitor of APC/C^CDH1 to start the cell cycle.

Nature

Cappell SD,Mark KG,Garbett D,Pack LR,Rape M,Meyer T

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Flow Cytometry

Fri Jun 01 00:00:00 UTC 2018

Progression through mitosis promotes PARP inhibitor-induced cytotoxicity in homologous recombination-deficient cancer cells.

Nature communications

Schoonen PM,Talens F,Stok C,Gogola E,Heijink AM,Bouwman P,Foijer F,Tarsounas M,Blatter S,Jonkers J,Rottenberg S,van Vugt MATM

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Mon Jul 17 00:00:00 UTC 2017

New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress.

Nucleic acids research

Ercilla A,Llopis A,Feu S,Aranda S,Ernfors P,Freire R,Agell N

37-6600 was used in western blot to study APC/C(Cdh1) function

Thu Jun 02 00:00:00 UTC 2016

New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress.

Nucleic acids research

Ercilla A,Llopis A,Feu S,Aranda S,Ernfors P,Freire R,Agell N

37-6600 was used in western blot to study APC/C(Cdh1) function

Thu Jun 02 00:00:00 UTC 2016

Feedback regulation between atypical E2Fs and APC/CCdh1 coordinates cell cycle progression.

EMBO reports

Boekhout M,Yuan R,Wondergem AP,Segeren HA,van Liere EA,Awol N,Jansen I,Wolthuis RM,de Bruin A,Westendorp B

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Tue Mar 01 00:00:00 UTC 2016

Hypersensitivity to DNA damage in antephase as a safeguard for genome stability.

Nature communications

Feringa FM,Krenning L,Koch A,van den Berg J,van den Broek B,Jalink K,Medema RH

37-6600 was used in western blot to describe a novel irreversible DNA-damage response in antephase

Fri Aug 26 00:00:00 UTC 2016

MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.

Biology open

Voets E,Wolthuis R

37-6600 was used in western blot to study the effect of MASTL on cyclin B1 degradation and its mechanism

Fri Mar 06 00:00:00 UTC 2015

Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles.

The Journal of cell biology

Kong D,Farmer V,Shukla A,James J,Gruskin R,Kiriyama S,Loncarek J

37-6600 was used in Western Blotting to study the role of Plk1 activity in the gradual, biochemical and structural maturation of centrioles.

Mon Sep 29 00:00:00 UTC 2014

Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis.

Virology

Yu Y,Munger K

37-6600 was used in western blot to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism

Fri Nov 01 00:00:00 UTC 2013

Selective enhancing effect of early mitotic inhibitor 1 (Emi1) depletion on the sensitivity of doxorubicin or X-ray treatment in human cancer cells.

The Journal of biological chemistry

Shimizu N,Nakajima NI,Tsunematsu T,Ogawa I,Kawai H,Hirayama R,Fujimori A,Yamada A,Okayasu R,Ishimaru N,Takata T,Kudo Y

Fri Jun 14 00:00:00 UTC 2013

The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress.

PloS one

Chen M,Gutierrez GJ,Ronai ZA

37-6600 was used in western blot to investigate the regulation and function of APC/C(Cdh) in non-genotoxic stress responses.

Mon Sep 10 00:00:00 UTC 2012

Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases.

The Journal of biological chemistry

Moshe Y,Bar-On O,Ganoth D,Hershko A

37-6600 was used in western blot to identify mechanisms other than degradation of Emi1, which release APC/C and promote entry into mitosis.

Fri May 13 00:00:00 UTC 2011

Substrate degradation by the anaphase promoting complex occurs during mitotic slippage.

Cell cycle (Georgetown, Tex.)

Lee J,Kim JA,Margolis RL,Fotedar R

Published figure using FBXO5 monoclonal antibody (Product # 37-6600) in Western Blot

Sat May 01 00:00:00 UTC 2010

Regulation of APC/C (Cdh1) ubiquitin ligase in differentiation of human embryonic stem cells.

Cell cycle (Georgetown, Tex.)

Bar-On O,Shapira M,Skorecki K,Hershko A,Hershko DD

37-6600 was used in Western Blotting to study the activity of APC/C (Cadh1) during the differentiation process of human embryonic stem cells.

Sat May 15 00:00:00 UTC 2010

DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest.

Molecular biology of the cell

Lee J,Kim JA,Barbier V,Fotedar A,Fotedar R

37-6600 was used in western blot to assess the role of p21(WAF1) in G2-M phase checkpoint control and in prevention of polyploidy after DNA damage

Wed Apr 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Early mitotic inhibitor 1; F-box only protein 5; F-box protein Fbx5

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Gene Aliases: EMI1; FBX5; Fbxo31; FBXO5

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UniProt ID: (Human) Q9UKT4

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Entrez Gene ID: (Human) 26271

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Function(s)

protein binding protein kinase binding metal ion binding

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle oocyte maturation spindle assembly involved in female meiosis I mitotic nuclear division regulation of mitotic nuclear division regulation of mitotic cell cycle vesicle organization negative regulation of meiotic nuclear division microtubule polymerization cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle

Performance Guarantee

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FBXO5 Monoclonal Antibody (3D2D6)

FBXO5 Antibody (37-6600) in WB

Product Details

37-6600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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