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Invitrogen
FBXO25 Polyclonal Antibody
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FIGURE: 1 / 1
FBXO25 Antibody (PA5-90255) in WB
Western blot analysis of FBXO25 in extracts of various cell lines. Samples were incubated with FBXO25 Polyclonal antibody (Product # PA5-90255) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-90255
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 142-291 of human FBXO25 (NP_0363052)
Conjugate
Form
Liquid
Concentration
1.65 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805985
Product Specific Information
Immunogen sequence: WQQQLQDLQM TKQVNNGLTL SDLPLHMLNN ILYRFSDGWD IITLGQVTPT LYMLSEDRQL WKKLCQYHFA EKQFCRHLIL SEKGHIEWKL MYFALQKHYP AKEQYGDTLH FCRHCSILFW KDSGHPCTAA DPDSCFTPVS PQHFIDLFKF; Positive Samples: DU145, 293T, Mouse testis; Cellular Location: Nucleus
Target Information
FBXO25 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO25 belongs to the Fbxs class.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: F-box only protein 25; F-box protein Fbx25; MGC20256; MGC51975
Gene Aliases: 9130015I06Rik; AI649137; FBX25; FBXO25
UniProt ID: (Human) Q8TCJ0, (Mouse) Q9D2Y6
Entrez Gene ID: (Human) 26260, (Mouse) 66822
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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