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Rockland
EZH1 Polyclonal Antibody
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FIGURE: 1 / 1
EZH1 Antibody (600-401-GT9) in WB
Western Blot of Rabbit anti-Ezh1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: NIH/3T3 WCL p/n W10-000-358. Lane 2: NIH/3T3 nuclear extract p/n W10-001-A74. Load: 10 µg per lane. Primary antibody: Ezh1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 60 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 86 kDa for Ezh1 in NIH/3T3 nuclear extract p/n W10-001-A74.
Product Details
600-401-GT9
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-EZH1 is directed against human EZH1 at an internal position. A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.
Target Information
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Enhancer of zeste homolog 1; ENX-2; Histone-lysine N-methyltransferase EZH1
Gene Aliases: ENX-2; Enx2; EZH1; KIAA0388; KMT6B
UniProt ID: (Human) Q92800, (Mouse) P70351
Entrez Gene ID: (Human) 2145, (Mouse) 14055
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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