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Invitrogen
E6AP Polyclonal Antibody
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FIGURE: 1 / 1
E6AP Antibody (PA1-24717) in WB
Western blot analysis of UBE3A using whole cell lysate from HeLa (5, 15 and 50µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50µg), and NIH-3T3 (M; 50µg) cells. Samples were probed with a UBE3A polyclonal antibody (Product # PA1-24717) at a dilution of 0.4µg/ml (A) and 1µg/ml (B) for WB and 3µg/mg lysate for IP. Detection was performed using chemiluminescence with exposure times of 3 minutes (A and B).
Product Details
PA1-24717
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a portion of human ubiquitin protein ligase E3A encoded within exon 7 (LocusLink ID 7337).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
PA1-24717 detects UBE3A from human samples. PA1-24717 is expected to cross react with mouse and Xenopus laevis due to sequence homology.
PA1-24717 has been successfully used in Western blot procedures.
The PA1-24717 immunogen is a synthetic peptide corresponding to a portion of human ubiquitin protein ligase E3A encoded within exon 7 (LocusLink ID 7337).
Target Information
UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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