Invitrogen

Dystrophin Polyclonal Antibody

Dystrophin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Dystrophin Antibody (PA5-16734) in IHC (P)

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Dystrophin antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of muscle fibers.

Dystrophin Antibody in Immunocytochemistry (ICC/IF)

Dystrophin Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-16734

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to C-terminal of human Dystrophin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10978300

Product Specific Information

PA5-16734 targets Dystrophin in IHC (P) applications and shows reactivity with Human and Rat samples.

The PA5-16734 immunogen is a synthetic peptide corresponding to C-terminal of human Dystrophin.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of vitamin A status on myogenic gene expression and muscle fiber characteristics.

Journal of animal science

Wellmann KB,Kim J,Urso PM,Smith ZK,Johnson BJ

PA5-16734 was used in Immunohistochemistry (Frozen) to indicate that gene expression associated with oxidative proteins may be independent of vitamin A status in yearling cattle.

Mon Mar 01 00:00:00 UTC 2021

Muscle phenotype of a rat model of Duchenne muscular dystrophy.

Muscle & nerve

Iyer SR,Xu S,Shah SB,Lovering RM

Published figure using Dystrophin polyclonal antibody (Product # PA5-16734) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

Muscle phenotype of a rat model of Duchenne muscular dystrophy.

Muscle & nerve

Iyer SR,Xu S,Shah SB,Lovering RM

Published figure using Dystrophin polyclonal antibody (Product # PA5-16734) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

A Novel Mutation in N-Terminal Actin-Binding Domain of the DMD Gene Presenting Becker Muscular Dystrophy as Recurrent Exertional Rhabdomyolysis: A Case Report.

Annals of Indian Academy of Neurology

Lee JM

Published figure using Dystrophin polyclonal antibody (Product # PA5-16734) in Immunohistochemistry

Mon Sep 28 00:00:00 UTC 2020

Co-Transplantation of Bone Marrow-MSCs and Myogenic Stem/Progenitor Cells from Adult Donors Improves Muscle Function of Patients with Duchenne Muscular Dystrophy.

Cells

Klimczak A,Zimna A,Malcher A,Kozlowska U,Futoma K,Czarnota J,Kemnitz P,Bryl A,Kurpisz M

PA5-16734 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to study the effect of a co-transplantation of two stem/progenitor cell populations (BM-MSCs and SM-SPCs) directly into the dystrophic muscle, on skeletal muscle function of Duchenne Muscular Dystrophy (DMD) patients.

Thu Apr 30 00:00:00 UTC 2020

Co-Transplantation of Bone Marrow-MSCs and Myogenic Stem/Progenitor Cells from Adult Donors Improves Muscle Function of Patients with Duchenne Muscular Dystrophy.

Cells

Klimczak A,Zimna A,Malcher A,Kozlowska U,Futoma K,Czarnota J,Kemnitz P,Bryl A,Kurpisz M

Thu Apr 30 00:00:00 UTC 2020

Acute and chronic resistance training downregulates select LINE-1 retrotransposon activity markers in human skeletal muscle.

American journal of physiology. Cell physiology

Romero MA,Mobley CB,Mumford PW,Roberson PA,Haun CT,Kephart WC,Healy JC,Beck DT,Young KC,Martin JS,Lockwood CM,Roberts MD

PA5-16734 was used in Immunohistochemistry-immunofluorescence to determine if acute or chronic resistance exercise affected markers of skeletal muscle long interspersed element-1 retrotransposon activity.

Thu Mar 01 00:00:00 UTC 2018

Targeted Expression of Catalase to Mitochondria Protects Against Ischemic Myopathy in High-Fat Diet-Fed Mice.

Diabetes

Ryan TE,Schmidt CA,Green TD,Spangenburg EE,Neufer PD,McClung JM

PA5-16734 was used in immunohistochemistry to study protection against ischemic myopathy in high fat fed mice by targeted expression of catalase to mitochondria

Thu Sep 01 00:00:00 UTC 2016

Recovery of altered neuromuscular junction morphology and muscle function in mdx mice after injury.

Cellular and molecular life sciences : CMLS

Pratt SJP,Shah SB,Ward CW,Kerr JP,Stains JP,Lovering RM

PA5-16734 was used in immunohistochemistry - frozen section to investigate mdx mice after injury and recovery of altered neuromuscular junction morphology and muscle function

Thu Jan 01 00:00:00 UTC 2015

The first exon duplication mouse model of Duchenne muscular dystrophy: A tool for therapeutic development.

Neuromuscular disorders : NMD

Vulin A,Wein N,Simmons TR,Rutherford AM,Findlay AR,Yurkoski JA,Kaminoh Y,Flanigan KM

PA5-16734 was used in western blot to generate and characterize a mouse model that carries a Dmd exon 2 duplication

Sun Nov 01 00:00:00 UTC 2015

Recovery of diaphragm function following mechanical ventilation in a rodent model.

PloS one

Bruells CS,Bergs I,Rossaint R,Du J,Bleilevens C,Goetzenich A,Weis J,Wiggs MP,Powers SK,Hein M

PA5-16734 was used in immunohistochemistry to use a rodent model to study the time-course of recovery of diaphragm muscle fiber atrophy and contractile dysfunction following return to voluntary breathing after mechanical ventilation

Mon Sep 29 00:00:00 UTC 2014

Obscurin is required for ankyrinB-dependent dystrophin localization and sarcolemma integrity.

The Journal of cell biology

Randazzo D,Giacomello E,Lorenzini S,Rossi D,Pierantozzi E,Blaauw B,Reggiani C,Lange S,Peter AK,Chen J,Sorrentino V

PA5-16734 was used in immunohistochemistry to characterize mice deficient for obscurin

Mon Feb 18 00:00:00 UTC 2013

Variable phenotype of del45-55 Becker patients correlated with nNOSμ mislocalization and RYR1 hypernitrosylation.

Human molecular genetics

Gentil C,Leturcq F,Ben Yaou R,Kaplan JC,Laforet P,Pénisson-Besnier I,Espil-Taris C,Voit T,Garcia L,Piétri-Rouxel F

PA5-16734 was used in immunohistochemistry to study the correlation of del45-55 Becker muscular dystrophy phenotype with nNOSmu mislocalization and RYR1 hypernitrosylation

Wed Aug 01 00:00:00 UTC 2012

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