Invitrogen

Dystrophin Polyclonal Antibody

Dystrophin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Dystrophin Antibody (PA1-21011) in IHC (P)

Immunohistochemical analysis of Dystrophin in paraffin-embedded mouse heart tissue using a Dystrophin polyclonal antibody (Product # PA1-21011) at a dilution of 1:50.

Dystrophin Antibody in Immunohistochemistry (IHC)

Dystrophin Antibody in Western Blot (WB)

Dystrophin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

PA1-21011

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Published species

Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to C-terminal of human Dystrophin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C, do not freeze

Shipping conditions

Wet ice

AB_2091358

Product Specific Information

PA1-21011 detects Dystrophin in Human, Mouse and Rat samples.

PA1-21011 has been successfully used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) immunocytochemistry, immunofluorescence, and Western Blot procedures. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Positive control of skeletal muscle suggested. Molecular weight of 427 kDa is expected.

PA1-21011 immunogen corresponds to a synthetic peptide corresponding to C-terminal of human Dystrophin.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vitamin D₃ supplementation does not enhance the effects of resistance training in older adults.

Journal of cachexia, sarcopenia and muscle

Mølmen KS,Hammarström D,Pedersen K,Lian Lie AC,Steile RB,Nygaard H,Khan Y,Hamarsland H,Koll L,Hanestadhaugen M,Eriksen AL,Grindaker E,Whist JE,Buck D,Ahmad R,Strand TA,Rønnestad BR,Ellefsen S

Published figure using Dystrophin polyclonal antibody (Product # PA1-21011) in Immunohistochemistry

Tue Jun 01 00:00:00 UTC 2021

Effects of ASC Application on Endplate Regeneration Upon Glycerol-Induced Muscle Damage.

Frontiers in molecular neuroscience

Rigon M,Hörner SJ,Straka T,Bieback K,Gretz N,Hafner M,Rudolf R

Published figure using Dystrophin polyclonal antibody (Product # PA1-21011) in Immunohistochemistry

Mon Sep 28 00:00:00 UTC 2020

The first exon duplication mouse model of Duchenne muscular dystrophy: A tool for therapeutic development.

Neuromuscular disorders : NMD

Vulin A,Wein N,Simmons TR,Rutherford AM,Findlay AR,Yurkoski JA,Kaminoh Y,Flanigan KM

PA1-21011 was used in immunohistochemistry - frozen section to generate and characterize a mouse model that carries a Dmd exon 2 duplication

Sun Nov 01 00:00:00 UTC 2015

Translation from a DMD exon 5 IRES results in a functional dystrophin isoform that attenuates dystrophinopathy in humans and mice.

Nature medicine

Wein N,Vulin A,Falzarano MS,Szigyarto CA,Maiti B,Findlay A,Heller KN,Uhlén M,Bakthavachalu B,Messina S,Vita G,Passarelli C,Brioschi S,Bovolenta M,Neri M,Gualandi F,Wilton SD,Rodino-Klapac LR,Yang L,Dunn DM,Schoenberg DR,Weiss RB,Howard MT,Ferlini A,Flanigan KM

PA1-21011 was used in immunohistochemistry to characterize attenuation of dystrophinopathy in humans and mice by translation from a DMD exon 5 IRES that results in a functional dystrophin isoform

Mon Sep 01 00:00:00 UTC 2014

Translation from a DMD exon 5 IRES results in a functional dystrophin isoform that attenuates dystrophinopathy in humans and mice.

Nature medicine

PA1-21011 was used in immunohistochemistry to characterize attenuation of dystrophinopathy in humans and mice by translation from a DMD exon 5 IRES that results in a functional dystrophin isoform

Mon Sep 01 00:00:00 UTC 2014

Variable t-tubule organization and Ca2+ homeostasis across the atria.

American journal of physiology. Heart and circulatory physiology

Frisk M,Koivumäki JT,Norseng PA,Maleckar MM,Sejersted OM,Louch WE

PA1-21011 was used in immunohistochemistry to study the effect of regional t-tubule density during cardiomyocyte Ca(2+) homeostasis

Fri Aug 15 00:00:00 UTC 2014

Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle.

The Journal of physiology

Sjåland C,Lunde PK,Swift F,Munkvik M,Ericsson M,Lunde M,Boye S,Christensen G,Ellingsen Ø,Sejersted OM,Andersson KB

PA1-21011 was used in immunohistochemistry to investigate the role of SERCAs in muscle contractile function

Thu Dec 15 00:00:00 UTC 2011

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