Invitrogen

DLC1 Polyclonal Antibody

DLC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DLC1 Antibody (PA5-28443) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Pheochromocytoma xenograft, using DLC1 (Product # PA5-28443) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

DLC1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Product Image Gallery

DLC1 Antibody — a The expression of DLC1 transcription variants in KT21 meningioma cells, qRT-PCR results. b Silencing of DLC1-v1 expression in KT21 cells through transfection with plasmid vector containing specific shRNA cassette as compared with cells transfected with control non-effective vector. Cells’ transfection efficacy assessed with flow cytometry, DLC1-v1 mRNA and protein level comparison between KT21 cells transfected with DCL1 specific (sh DLC1-v1) and non-specific shRNA vectors sh control. c Active RHO-GTPases pull-down assay in KT21 cells transfected with shRNA targeting DLC1 and control cells. RHO-GTPases were detected with antibody specific to RhoA, RhoB and RhoC. d Result of MTT test performed in KT21 cells transfected with shRNA targeting DLC1 and control cells. e The results of woundhealing assay performed in KT21 cells transfected with shRNA targeting DLC1 and control cells. The examplary fields marked for the measurement of the migration distance. The comparison of migration distance of shDLC1-1 and sh-control KT21 cells. {KD}

Product Details

PA5-28443

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 43 of Human DLC1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545919

Product Specific Information

Recommended positive controls: H1299, HeLaS3, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (100%), Dog (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a GTPase-activating protein that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant DNA methylation of alternative promoter of DLC1 isoform 1 in meningiomas.

Journal of neuro-oncology

Bujko M,Kober P,Rusetska N,Wakuła M,Goryca K,Grecka E,Matyja E,Neska J,Mandat T,Bonicki W,Siedlecki JA

PA528443 was used in immunohistochemistry - paraffin section and western blot to examine methylation of the DLC1 isoform 1 alternative promoter in normal and meningioma FFPE samples

Thu Dec 01 00:00:00 UTC 2016