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Invitrogen
DIAPH2 Monoclonal Antibody (V78 P3C10-D3), APC
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FIGURE: 1 / 1
DIAPH2 Antibody (MA5-45918) in WB
Western Blot analysis of Mouse liver showing detection of 125 kda DIAPH2 protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver (10 µg). Load: 10 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with DIAPH2 monoclonal antibody (Product # MA5-45918) at 1:1,000 for 2 hours at RT with shaking, followed by Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 125 kda.
Product Details
MA5-45918
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
V78 P3C10-D3
Immunogen
Synthetic peptide of Human DIAPH2
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A 1:1,000 dilution of MA5-45918 was sufficient for detection of DIAPH2 in 10 µg of Mouse Liver by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 125 kDa.
Target Information
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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