Invitrogen

Cyclin D2 Monoclonal Antibody (DCS3.1)

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Cyclin D2 Antibody (MA1-12297) in WB

Journal of diabetes investigation 2019 - Figure 7 Insulin secretion and change in messenger ribonucleic acid expression levels in MIN 6-K8 beta-cells. (a) Effects of glucose, glimepiride and nifedipine on insulin secretion in MIN 6-K8 beta-cells. Insulin secretion from MIN 6-K8 beta-cells was normalized by cellular insulin content ( n = 7 per group). (b, c) Messenger ribonucleic acid expression levels of cyclinD2 and Irs2 in MIN 6-K8 beta-cells ( ... View More

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Product Details

MA1-12297

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Avian, Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

DCS3.1

Immunogen

His-tagged recombinant full-length Cyclin D2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2275323

Target Information

Cyclin D2 is a G1 cyclin required for G1 phase progression and is a strong candidate for a proto-oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass.

Journal of diabetes investigation

Murase M,Seino Y,Maekawa R,Iida A,Hosokawa K,Hayami T,Tsunekawa S,Hamada Y,Yokoi N,Seino S,Hayashi Y,Arima H

MA1-12297 was used in Western Blotting to investigate the role of a high-carbohydrate diet on Β-cell mass (BCM) in mice.

Fri Mar 01 00:00:00 UTC 2019

Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass.

Journal of diabetes investigation

Murase M,Seino Y,Maekawa R,Iida A,Hosokawa K,Hayami T,Tsunekawa S,Hamada Y,Yokoi N,Seino S,Hayashi Y,Arima H

MA1-12297 was used in Western Blotting to investigate the role of a high-carbohydrate diet on Β-cell mass (BCM) in mice.

Fri Mar 01 00:00:00 UTC 2019

Sustained expression of the transcription factor GLIS3 is required for normal beta cell function in adults.

EMBO molecular medicine

Yang Y,Chang BH,Chan L

MA1-12297 was used in western blot to study the role of the GLIS3 transcription factor in normal pancreatic beta-cell maintenance and proliferation in response to a high fat diet in adult mice

Tue Jan 01 00:00:00 UTC 2013

Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression.

Cancer research

Zhang Q,Sakamoto K,Liu C,Triplett AA,Lin WC,Rui H,Wagner KU

MA1-12297 was used in immunoprecipitation and western blot to study the ability of cyclin D3 to compensate for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression

Thu Dec 15 00:00:00 UTC 2011

Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression.

Cancer research

Zhang Q,Sakamoto K,Liu C,Triplett AA,Lin WC,Rui H,Wagner KU

MA1-12297 was used in immunoprecipitation and western blot to study the ability of cyclin D3 to compensate for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression

Thu Dec 15 00:00:00 UTC 2011

GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).

Islets

Stein J,Milewski WM,Hara M,Steiner DF,Dey A

MA1-12297 was used in western blot to study the mechanism by which GSK-3 inactivation or depletion promotes beta-cell replication

Tue Jun 07 00:00:00 UTC 2011

Control of cyclin D1 and breast tumorigenesis by the EglN2 prolyl hydroxylase.

Cancer cell

Zhang Q,Gu J,Li L,Liu J,Luo B,Cheung HW,Boehm JS,Ni M,Geisen C,Root DE,Polyak K,Brown M,Richardson AL,Hahn WC,Kaelin WG,Bommi-Reddy A

MA1-12297 was used in western blot to study the role of EglN2 prolyl hydroxylase in breast carcinogenesis

Fri Nov 06 00:00:00 UTC 2009

Adaptive beta-cell proliferation is severely restricted with advanced age.

Diabetes

Rankin MM,Kushner JA

MA1-12297 was used in western blot to study the impact of ageing on adaptive pancreatic beta-cell proliferation

Mon Jun 01 00:00:00 UTC 2009

Cyclin D2 protein stability is regulated in pancreatic beta-cells.

Molecular endocrinology (Baltimore, Md.)

He LM,Sartori DJ,Teta M,Opare-Addo LM,Rankin MM,Long SY,Diehl JA,Kushner JA

MA1-12297 was used in immunohistochemistry and western blot to study the regulation of cyclin D2 protein stability in pancreatic beta-cells

Sun Nov 01 00:00:00 UTC 2009

Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine.

Cancer biology & therapy

Zheng Y,Bie W,Yang R,Perekatt AO,Poole AJ,Tyner AL

MA1-12297 was used in western blot to investigate the role of p21 and p27 in intestinal epithelial regeneraton

Sun Jun 01 00:00:00 UTC 2008

Molecular characterization of spontaneous mesenchymal stem cell transformation.

PloS one

Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A

MA1-12297 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation

Wed Jan 02 00:00:00 UTC 2008

Selective cortical interneuron and GABA deficits in cyclin D2-null mice.

Development (Cambridge, England)

Glickstein SB,Moore H,Slowinska B,Racchumi J,Suh M,Chuhma N,Ross ME

MA1-12297 was used in immunohistochemistry to study selective cortical interneuron and GABA deficits in cyclin D2-null mice

Thu Nov 01 00:00:00 UTC 2007

Effects of granulosa cell-specific deletion of Rb in Inha-alpha null female mice.

Endocrinology

Andreu-Vieyra C,Chen R,Matzuk MM

MA1-12297 was used in western blot to investigate the role of RB protein in ovarian tumorigenesis

Wed Aug 01 00:00:00 UTC 2007

Loss of cyclin D1 impairs cerebellar development and suppresses medulloblastoma formation.

Development (Cambridge, England)

Pogoriler J,Millen K,Utset M,Du W

MA1-12297 was used in immunohistochemistry to study the role of cyclin D1 in cerebellar development and medulloblastoma formation

Sun Oct 01 00:00:00 UTC 2006

p75NTR enhances PC12 cell tumor growth by a non-receptor mechanism involving downregulation of cyclin D2.

Experimental cell research

Fritz MD,Mirnics ZK,Nylander KD,Schor NF

MA1-12297 was used in western blot to elucidate how p75NTR suppresses cyclin D2 expression in PC12 cells

Sun Oct 15 00:00:00 UTC 2006

Pomegranate fruit juice for chemoprevention and chemotherapy of prostate cancer.

Proceedings of the National Academy of Sciences of the United States of America

Malik A,Afaq F,Sarfaraz S,Adhami VM,Syed DN,Mukhtar H

MA1-12297 was used in western blot to investigate the protective effect of pomegranate fruit juice against prostate cancer

Tue Oct 11 00:00:00 UTC 2005

Cyclin D2 compensates for the loss of cyclin D1 in estrogen-induced mouse uterine epithelial cell proliferation.

Molecular endocrinology (Baltimore, Md.)

Chen B,Pollard JW

MA1-12297 was used in immunohistochemistry and western blot to study the ability of cyclin D2 to compensate for cyclin D1 loss in estrogen-induced mouse uterine epithelial cell proliferation

Tue Jul 01 00:00:00 UTC 2003

Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells.

Oncogene

Gupta S,Afaq F,Mukhtar H

MA1-12297 was used in western blot to investigate apigenin-induced apoptosis in human prostate cancer cells

Thu May 23 00:00:00 UTC 2002

v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.

Molecular and cellular biology

Clark W,Black EJ,MacLaren A,Kruse U,LaThangue N,Vogt PK,Gillespie DA

MA1-12297 was used in immunoprecipitation to study the mechanism by which v-Jun promotes S-phase entry in the absence of mitogens

Sat Apr 01 00:00:00 UTC 2000

Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.

Molecular and cellular biology

Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H

MA1-12297 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown

Fri Oct 01 00:00:00 UTC 1999

Regulation of exit from quiescence by p27 and cyclin D1-CDK4.

Molecular and cellular biology

Ladha MH,Lee KY,Upton TM,Reed MF,Ewen ME

MA1-12297 was used in western blot to study the role of p27 and cyclin D1-CDK4 in regulating S-phase entry of quiescent NIH 3T3 cells

Sun Nov 01 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: G1/S-specific cyclin-D2; MGC102758; Vin-1 proto-oncogene

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Gene Aliases: 2600016F06Rik; AI256817; BF642806; C86853; CCND2; cD2; Cyl-2; KIAK0002; MPPH3; Vin-1; Vin1

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UniProt ID: (Human) P30279, (Rat) Q04827, (Mouse) P30280

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Entrez Gene ID: (Human) 894, (Rat) 64033, (Mouse) 12444

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Function(s)

protein binding protein kinase binding

Process(es)

positive regulation of protein phosphorylation cell cycle positive regulation of cyclin-dependent protein serine/threonine kinase activity cell division G1/S transition of mitotic cell cycle ovarian follicle development liver development spermatogenesis positive regulation of cell proliferation response to organic cyclic compound response to estradiol cellular response to insulin stimulus response to testosterone response to peptide hormone response to ethanol regulation of neuron differentiation positive regulation of epithelial cell proliferation response to cAMP regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cyclin D2 Monoclonal Antibody (DCS3.1)

Cyclin D2 Antibody (MA1-12297) in WB

Product Details

MA1-12297

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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