Invitrogen

Cyclin D1 Polyclonal Antibody

Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)

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Cyclin D1 Antibody (PA5-16232) in ICC/IF

Immunofluorescence analysis of Cyclin D1 / Bcl-1 was performed using 70% confluent MCF-7 cells serum starved for 16h followed by 2h release. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin D1 Rabbit Polyclonal Antibody (Product # PA5-16232) at 2µg/mli... View More

Cyclin D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclin D1 Antibody in Immunoprecipitation (IP)

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Product Details

PA5-16232

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

10 µg/mL

Product Specifications

Published species

Fish, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from the C-terminus of human cyclin D1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_10979663

Product Specific Information

PA5-16232 targets Cyclin D1/Bcl-1 in IF, IP, and WB applications and shows reactivity with mouse, Rat, and Human samples.

The PA5-16232 immunogen is a synthetic peptide derived from the C-terminus of human cyclin D1.

Target Information

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDK4 is an essential insulin effector in adipocytes.

The Journal of clinical investigation

Lagarrigue S,Lopez-Mejia IC,Denechaud PD,Escoté X,Castillo-Armengol J,Jimenez V,Chavey C,Giralt A,Lai Q,Zhang L,Martinez-Carreres L,Delacuisine B,Annicotte JS,Blanchet E,Huré S,Abella A,Tinahones FJ,Vendrell J,Dubus P,Bosch F,Kahn CR,Fajas L

PA5-16232 was used in western blot to characterize an essential insulin effector in adipocytes called CDK4

Fri Jan 01 00:00:00 UTC 2016

Hyper sensitive protein detection by Tandem-HTRF reveals Cyclin D1 dynamics in adult mouse.

Scientific reports

Zampieri A,Champagne J,Auzemery B,Fuentes I,Maurel B,Bienvenu F

PA5-16232 was used in western blot to reveal Cyclin D1 dynamics in adult mice by hyper sensitive protein detection by tandem-HTRF

Tue Oct 27 00:00:00 UTC 2015

Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase.

Genes & development

Zheng X,Zhai B,Koivunen P,Shin SJ,Lu G,Liu J,Geisen C,Chakraborty AA,Moslehi JJ,Smalley DM,Wei X,Chen X,Chen Z,Beres JM,Zhang J,Tsao JL,Brenner MC,Zhang Y,Fan C,DePinho RA,Paik J,Gygi SP,Kaelin WG,Zhang Q

PA5-16232 was used in western blot to elucidate how EglN2 regulates FOXO3a degradation.

Tue Jul 01 00:00:00 UTC 2014

Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase.

Genes & development

PA5-16232 was used in western blot to elucidate how EglN2 regulates FOXO3a degradation.

Tue Jul 01 00:00:00 UTC 2014

Cyclin D1 and C/EBPβ LAP1 operate in a common pathway to promote mammary epithelial cell differentiation.

Molecular and cellular biology

Liu Q,Boudot A,Ni J,Hennessey T,Beauparlant SL,Rajabi HN,Zahnow C,Ewen ME

PA5-16232 was used in ChIP assay and western blot to investigate the relationship between cyclin D1 and the transcription factor C/EBPbeta in mammary epithelial cell differentiation

Fri Aug 01 00:00:00 UTC 2014

Low-oxygen culture conditions extend the multipotent properties of human retinal progenitor cells.

Tissue engineering. Part A

Baranov PY,Tucker BA,Young MJ

PA5-16232 was used in immunohistochemistry to study the extension of human retinal progenitor cell multipotency and self-renewal by culture under conditions of low oxygen

Thu May 01 00:00:00 UTC 2014

p27 is regulated independently of Skp2 in the absence of Cdk2.

Biochimica et biophysica acta

Kotoshiba S,Gopinathan L,Pfeiffenberger E,Rahim A,Vardy LA,Nakayama K,Nakayama KI,Kaldis P

PA5-16232 was used in western blot to use knockout mice to study the Skp2-independent modulation of p27 in Cdk2 ablated mice

Sat Feb 01 00:00:00 UTC 2014

A melanocyte lineage program confers resistance to MAP kinase pathway inhibition.

Nature

Johannessen CM,Johnson LA,Piccioni F,Townes A,Frederick DT,Donahue MK,Narayan R,Flaherty KT,Wargo JA,Root DE,Garraway LA

PA5-16232 was used in western blot to study MAPK pathway inhibition resistance in malignant melanoma and the role of melanocyte lineage dysregulation

Thu Dec 05 00:00:00 UTC 2013

Dynamin inhibitors impair endocytosis and mitogenic signaling of PDGF.

Traffic (Copenhagen, Denmark)

Sadowski Ł,Jastrzębski K,Kalaidzidis Y,Heldin CH,Hellberg C,Miaczynska M

PA5-16232 was used in immunocytochemistry to observe internalization and endosomal trafficking of platelet-derived growth factor in human fibroblasts

Sat Jun 01 00:00:00 UTC 2013

The oxysterol receptors LXRα and LXRβ suppress proliferation in the colon.

Molecular carcinogenesis

Vedin LL,Gustafsson JÅ,Steffensen KR

PA5-16232 was used in western blot to study the role of oxysterol receptors LXRalpha and LXRbeta in suppressing proliferation in the colon

Fri Nov 01 00:00:00 UTC 2013

The widely used Wnt1-Cre transgene causes developmental phenotypes by ectopic activation of Wnt signaling.

Developmental biology

Lewis AE,Vasudevan HN,O'Neill AK,Soriano P,Bush JO

PA5-16232 was used in immunohistochemistry to evaluate the Wnt1-Cre transgenic mouse line as a model for the study of midbrain development

Mon Jul 15 00:00:00 UTC 2013

Cyclin D1 induction of Dicer governs microRNA processing and expression in breast cancer.

Nature communications

Yu Z,Wang L,Wang C,Ju X,Wang M,Chen K,Loro E,Li Z,Zhang Y,Wu K,Casimiro MC,Gormley M,Ertel A,Fortina P,Chen Y,Tozeren A,Liu Z,Pestell RG

PA5-16232 was used in western blot to study miRNA expression and processing in breast cancer and the regulatory role of cyclin D1 induction of Dicer expression

Tue Jul 15 00:00:00 UTC 2014

Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.

The Journal of biological chemistry

Kundumani-Sridharan V,Singh NK,Kumar S,Gadepalli R,Rao GN

PA5-16232 was used in western blot to study the roles of NFATc1 and Pak1 in chemokine-induced aortic smooth muscle proliferation and vascular remodeling following injury

Fri Jul 26 00:00:00 UTC 2013

A microRNA-21 surge facilitates rapid cyclin D1 translation and cell cycle progression in mouse liver regeneration.

The Journal of clinical investigation

Ng R,Song G,Roll GR,Frandsen NM,Willenbring H

PA5-16232 was used in immunohistochemistry to study the role of enhanced cyclin D1 translation in the mechanism by which miRNA-21 promotes liver regeneration

Thu Mar 01 00:00:00 UTC 2012

Apoptosis signal-regulating kinase-1 inhibitor as a potent therapeutic drug for the treatment of gastric cancer.

Cancer science

Hayakawa Y,Hirata Y,Sakitani K,Nakagawa H,Nakata W,Kinoshita H,Takahashi R,Takeda K,Ichijo H,Maeda S,Koike K

PA5-16232 was used in immunohistochemistry to study the therapeutic potential of ASK1 inhibition in gastric cancer

Sat Dec 01 00:00:00 UTC 2012

CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.

Cell cycle (Georgetown, Tex.)

McClendon AK,Dean JL,Rivadeneira DB,Yu JE,Reed CA,Gao E,Farber JL,Force T,Koch WJ,Knudsen ES

PA5-16232 was used in western blot to study the protective effect of pharmacological inhibition of CDK4/6 against antracycline cytotoxicity in triple-negative breast cancer

Sun Jul 15 00:00:00 UTC 2012

Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells.

Molecular and cellular biology

Sarkar TR,Sharan S,Wang J,Pawar SA,Cantwell CA,Johnson PF,Morrison DK,Wang JM,Sterneck E

PA5-16232 was used in western blot to study the role of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway in the transformation of breast tumor cells

Sun Jan 01 00:00:00 UTC 2012

Cyclin D1 inactivation extends proliferation and alters histogenesis in the postnatal mouse retina.

Developmental dynamics : an official publication of the American Association of Anatomists

Das G,Clark AM,Levine EM

PA5-16232 was used in immunohistochemistry to study post-natal murine retinal histogenesis and the role played by cyclin D1

Tue May 01 00:00:00 UTC 2012

Cell cycle arrest associated with anoxia-induced quiescence, anoxic preconditioning, and embryonic diapause in embryos of the annual killifish Austrofundulus limnaeus.

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology

Meller CL,Meller R,Simon RP,Culpepper KM,Podrabsky JE

PA5-16232 was used in western blot to study the role of cell cycle arrest in Austrofundulus limnaeus embryonic diapause

Mon Oct 01 00:00:00 UTC 2012

Novel interactions between NFATc1 (Nuclear Factor of Activated T cells c1) and STAT-3 (Signal Transducer and Activator of Transcription-3) mediate G protein-coupled receptor agonist, thrombin-induced biphasic expression of cyclin D1, with first phase influencing cell migration and second phase directing cell proliferation.

The Journal of biological chemistry

Kundumani-Sridharan V,Van Quyen D,Subramani J,Singh NK,Chin YE,Rao GN

PA5-16232 was used in immunocytochemistry to study the role of NFATc1 and STAT3 in mediating the expression of cyclin D1

Fri Jun 29 00:00:00 UTC 2012

Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices.

International journal of hepatology

Finot F,Masson R,Desmots F,Ribault C,Bichet N,Vericat JA,Lafouge P,Guguen-Guillouzo C,Loyer P

PA5-16232 was used in western blot to study the proliferation of liver slice hepatocytes in response to combined stimulation with TNF-alpha and EGF

Mon Nov 05 00:00:00 UTC 2012

Modification of the DNA damage response by therapeutic CDK4/6 inhibition.

The Journal of biological chemistry

Dean JL,McClendon AK,Knudsen ES

PA5-16232 was used in western blot to study the effects of therapeutic CDK4/6 inhibition on the DNA damage response and the clinical implications

Fri Aug 17 00:00:00 UTC 2012

Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.

The Journal of biological chemistry

Singh NK,Kundumani-Sridharan V,Kumar S,Verma SK,Kotla S,Mukai H,Heckle MR,Rao GN

PA5-16232 was used in western blot to study the role of protein kinase N1 in NFADc1-dependent cyclin D1/CDK6 expression and activity and the significance for remodeling of inner bood vessel walls

Fri Oct 19 00:00:00 UTC 2012

MUC1-C oncoprotein induces TCF7L2 transcription factor activation and promotes cyclin D1 expression in human breast cancer cells.

The Journal of biological chemistry

Rajabi H,Ahmad R,Jin C,Kosugi M,Alam M,Joshi MD,Kufe D

PA5-16232 was used in western blot to study the role of TCF7L2 activation in the mechanism by which oncogenic MUC1-C promotes human breast cancer cell cyclin D1 expression

Fri Mar 23 00:00:00 UTC 2012

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Cyclin D1 Polyclonal Antibody

Cyclin D1 Antibody (PA5-16232) in ICC/IF

Product Details

PA5-16232

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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