Invitrogen

Claudin 3 Polyclonal Antibody

Claudin 3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Claudin 3 Antibody (PA1-37469) in ICC/IF

Immunofluorescence analysis of Claudin-3 was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Claudin 3 Polyclonal Antibody (Product # PA1-37469, PA5-32353) at 1:100 dilution in 0.1% BSA, incubated... View More

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Product Details

PA1-37469

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminus of mouse Claudin-3

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2082840

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is small intestine tissue.

Target Information

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRPV6 channel mediates alcohol-induced gut barrier dysfunction and systemic response.

Cell reports

Meena AS,Shukla PK,Bell B,Giorgianni F,Caires R,Fernández-Peña C,Beranova S,Aihara E,Montrose MH,Chaib M,Makowski L,Neeli I,Radic MZ,Vásquez V,Jaggar JH,Cordero-Morales JF,Rao R

PA5-32353 was used in Immunohistochemistry-immunofluorescence to identify TRPV6, a Ca2+-permeable channel, as responsible for alcohol-induced elevation of intracellular Ca2+, intestinal barrier dysfunction, and systemic inflammation.

Tue Jun 14 00:00:00 UTC 2022

Disruption of the pro-inflammatory, anti-inflammatory cytokines and tight junction proteins expression, associated with changes of the composition of the gut microbiota in patients with irritable bowel syndrome.

PloS one

Ivashkin V,Poluektov Y,Kogan E,Shifrin O,Sheptulin A,Kovaleva A,Kurbatova A,Krasnov G,Poluektova E

PA5-32353 was used in Immunohistochemistry to assess the changes of gut microbiome composition, tight junction proteins, and cytokines expression of intestinal mucosa from the duodenum to the distal part of the colon in irritable bowel syndrome patients and healthy volunteers.

Tue Nov 16 00:00:00 UTC 2021