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OriGene
Cas9 Monoclonal Antibody (OTI1C8), TrueMAB™
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FIGURE: 1 / 1
Cas9 Antibody (TA811327) in IP
Immunoprecipitation(IP) of Cas9 and Cas9-nickase by using mouse monoclonal anti-CAS9 antibodies TA811194 and TA811327. Mouse IgG control serves as the negative control. 293T cells were transfected with flag-tagged Cas9 overexpression plasmid, pCas-Guide(G) and pCas-Guide-nickase(N). Overexpression cell lysates were first precleared with ProteinG beads then incubated with beads crosslinked with anti-CAS9 antibody for overnight. The beads were then rinced with buffer and went thro µgh Western Blot analysis using anti-flag antibody (TA180144)... View More
Product Details
TA811327
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1C8
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-1166 of human CAS9 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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