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Invitrogen
Carbonic Anhydrase IX Polyclonal Antibody
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FIGURE: 1 / 4
Carbonic Anhydrase IX Antibody (PA5-19441) in WB
Western blot was performed using Anti-Carbonic Anhydrase IX Polyclonal Antibody (Product # PA5-19441) and ~ 50-55 kDa bands corresponding to CA9 was observed across cell lines tested and increased upon Deferoxamine and Cobalt Chloride treatment in HeLa along with an uncharacterized band (*) at ~35 kDa. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HeLa (Lane 2), HeLa treated with Deferoxamine ... View More
Product Details
PA5-19441
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Carbonic Anhydrase IX.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10982975
Product Specific Information
Heat mediated antigen retrieval recommended prior to tissue staining.
Target Information
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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