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Invitrogen
Calpain S1/S2 Monoclonal Antibody (28F3)
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Calpain S1/S2 Antibody (MA1-34966) in WB
Western blot was performed using Anti-Calpain S1/S2 Monoclonal Antibody (28F3) (Product # MA1-34966) and a 28kDa band corresponding to Calpain S1/S2 was observed across the tested cell lines, except Jurkat and NIH/3T3. Whole Cell Extract-TEB (30 µg lysate) of MCF7 (Lane 1), IMR-32 (Lane 2), Jurkat (Lane 3), DU 145 (Lane 4), NIH/3T3 (Lane 5), A-431 (Lane 6), and PC-3 (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB... View More
Product Details
MA1-34966
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
28F3
Immunogen
Full length protein (Human). Antibody specifically recognizes small subunit.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.5, with 50% glycerol
Contains
3mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2069488
Product Specific Information
This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). It recognizes both native and denatured protein in ELISA, Western Blot and Immunoprecipitation.
Target Information
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and m-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, m-calpain is active at micromolar (mM) concentrations of Ca++. In addition to the ubiquitously expressed m- and m- calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calcium-activated neutral proteinase small subunit; Calcium-dependent protease small subunit; Calcium-dependent protease small subunit 1; Calcium-dependent protease small subunit 2; calcium-dependent protease, small subunit; calpain 4, small subunit (30K); Calpain regulatory subunit; Calpain small subunit 1; Calpain small subunit 2; calpain, small polypeptide; calpain, small subunit 1; calpain, small subunit 2; CANP small subunit; CDPS; CSS1; CSS2
Gene Aliases: BOS_17486; CALPAIN4; CANP; CANPS; CAPN4; CAPNS; CAPNS1; CAPNS2; CDPS; CSS1
UniProt ID: (Human) P04632, (Bovine) P13135, (Human) Q96L46
Entrez Gene ID: (Human) 826, (Bovine) 281664, (Human) 84290
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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