Invitrogen

Calcium Sensing Receptor Polyclonal Antibody

Calcium Sensing Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Calcium Sensing Receptor Antibody (PA1-37213) in IHC (P)

Immunohistochemical (paraffin) analysis of Calcium Sensing Receptor using a polyclonal antibody (Product # PA1-37213) in human placenta tissue at a dilution of 1:50.

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Product Details

PA1-37213

Applications

Tested Dilution

Publications

Western Blot (WB)

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from N-terminus of rat calcium sensing receptor

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2071481

Product Specific Information

This antibody is predicted to react with bovine and mouse based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.

Target Information

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G- protein that activates a phosphatidylinositol-calcium second messenger system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High Salt Upregulates Ca²⁺-Sensing Receptor Expression and Ca²⁺-Induced Relaxation of Contracted Mesenteric Arteries from Dahl Salt-Sensitive Rats.

The Journal of pharmacology and experimental therapeutics

Bridges LE,Williams CL,Awumey EM

PA1-37213 was used in Western Blot to conclude that up-regulated expression of the CaSR in salt sensitivity increased receptor-mediated vascular relaxation in rats.

Sun May 01 00:00:00 UTC 2022

Notch enhances Ca²⁺ entry by activating calcium-sensing receptors and inhibiting voltage-gated K⁺ channels.

American journal of physiology. Cell physiology

Song S,Babicheva A,Zhao T,Ayon RJ,Rodriguez M,Rahimi S,Balistrieri F,Harrington A,Shyy JY,Thistlethwaite PA,Makino A,Yuan JX

PA1-37213 was used in Western Blot to suggest that Notch activation enhances CaSR-mediated increases in [Ca2+]cyt by enhancing store-operated Ca2+ entry and inhibits KCNA5/KV1.5 and KCNA2/KV1.2, ultimately leading to voltage-activated Ca2+ entry.

Fri May 01 00:00:00 UTC 2020

Immunohistochemical Predictors of Bone Metastases in Breast Cancer Patients.

Pathology oncology research : POR

Winczura P,Sosińska-Mielcarek K,Duchnowska R,Badzio A,Lakomy J,Majewska H,Pęksa R,Pieczyńska B,Radecka B,Dębska-Szmich S,Adamowicz K,Biernat W,Jassem J

PA1-37213 was used in immunohistochemistry to evaluate the potential protein markers of bone metastases in breast cancer patients

Tue Sep 01 00:00:00 UTC 2015

Gαi/o-dependent Ca(2+) mobilization and Gαq-dependent PKCα regulation of Ca(2+)-sensing receptor-mediated responses in N18TG2 neuroblastoma cells.

Neurochemistry international

Sesay JS,Gyapong RN,Najafi LT,Kabler SL,Diz DI,Howlett AC,Awumey EM

PA1-37213 was used in Western Blot to indicate that the CaS-mediated increase in [Ca(2+)]i in N18TG2 cells is dependent on Gαi/o proteins via inositol-1,4,5-triphosphate (IP3) channels and store-operated Ca(2+) entry channels, whereas modulation of CaS responses involving PKCα phosphorylation and translocation to the plasma membrane occurs via a Gαq mechanism.

Sun Nov 01 00:00:00 UTC 2015

A novel loss-of-function mutation, Gln459Arg, of the calcium-sensing receptor gene associated with apparent autosomal recessive inheritance of familial hypocalciuric hypercalcemia.

The Journal of clinical endocrinology and metabolism

Lietman SA,Tenenbaum-Rakover Y,Jap TS,Yi-Chi W,De-Ming Y,Ding C,Kussiny N,Levine MA

PA1-37213 was used in western blot to identify novel loss-of-function Q459R mutation in the CASR gene and to study its effect on autosomal dominant familial hypocalciuric hypercalcemia.

Sun Nov 01 00:00:00 UTC 2009

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